ArticleCancer management and research2018
Probe-based three-dimensional confocal laser endomicroscopy of brain tumors: technical note.
Article in Cancer management and research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Fluorescein-stained confocal laser endomicroscopy versus conventional frozen section for intraoperative histopathological assessment of intracranial tumors.Neuro-oncology · 2024Trial
- Real-timeFrontiers in oncology · 2026Article
- Towards Optical Biopsy in Glioma Surgery.International journal of molecular sciences · 2025Review
- Confocal Laser Endomicroscopy: Enhancing Intraoperative Decision Making in Neurosurgery.Diagnostics (Basel, Switzerland) · 2025Review
- Innovative Damage Assessment of Endodontic Instruments Based on Digital Image Stacking.Clinics and practice · 2024Article
- A prospective multicenter assessor blinded pilot study using confocal laser endomicroscopy for intraoperative brain tumor diagnosis.Scientific reports · 2024Article
- A Handheld Visible Resonance Raman Analyzer Used in Intraoperative Detection of Human Glioma.Cancers · 2023Article
- 5-aminolevulinic acid, fluorescein sodium, and indocyanine green for glioma margin detection: analysis of operating wide-field and confocal microscopy in glioma models of various grades.Frontiers in oncology · 2023Article
- Development, Implementation and Application of Confocal Laser Endomicroscopy in Brain, Head and Neck Surgery-A Review.Diagnostics (Basel, Switzerland) · 2022Review
- OCT-Guided Surgery for Gliomas: Current Concept and Future Perspectives.Diagnostics (Basel, Switzerland) · 2022Review
- Characterization ofFrontiers in oncology · 2022Article
- Confocal laser imaging in neurosurgery: A comprehensive review of sodium fluorescein-based CONVIVO preclinical and clinical applications.Frontiers in oncology · 2022Review
- Confocal Laser Microscopy in Neurosurgery: State of the Art of Actual Clinical Applications.Journal of clinical medicine · 2021Review
- Soliton microcomb based spectral domain optical coherence tomography.Nature communications · 2021Article
- Redosing of Fluorescein Sodium Improves Image Interpretation During IntraoperativeFrontiers in oncology · 2021Article
- Fluorescein-Guided Panendoscopy for Head and Neck Cancer Using Handheld Probe-Based Confocal Laser Endomicroscopy: A Pilot Study.Frontiers in oncology · 2021Article
- Confocal Laser Endomicroscopy Assessment of Pituitary Tumor Microstructure: A Feasibility Study.Journal of clinical medicine · 2020Article
- Compact and contactless reflectance confocal microscope for neurosurgery.Biomedical optics express · 2020Article
- Article
- Video-Mosaicked Handheld Dual-Axis Confocal Microscopy of Gliomas: AnFrontiers in oncology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConfocal laser endomicroscopy (CLE) is used during fluorescence-guided brain tumor surgery for intraoperative microscopy of tumor tissue with cellular resolution. CLE could augment and expedite intraoperative decision-making and potentially aid in diagnosis and removal of tumor tissue.
objectiveTo describe an extension of CLE imaging modality that produces Z-stack images and three-dimensional (3D) pseudocolored volumetric images. MATERIALS AND
methodsHand-held probe-based CLE was used to collect images from GL261-luc2 gliomas in C57BL/6 mice and from human brain tumor biopsies. The mice were injected with fluorescein sodium (FNa) before imaging. Patients received FNa intraoperatively, and biopsies were imaged immediately in the operating room. Some specimens were counterstained with acridine orange, acriflavine, or Hoechst and imaged on a benchtop confocal microscope. CLE images at various depths were acquired automatically, compiled, rendered into 3D volumes using Fiji software and reviewed by a neuropathologist and neurosurgeons.
resultsCLE imaging, Z-stack acquisition, and 3D image rendering were performed using 19 mouse gliomas and 31 human tumors, including meningiomas, gliomas, and pituitary adenomas. Volumetric images and Z-stacks provided additional information about fluorescence signal distribution, cytoarchitecture, and the course of abnormal vasculature.
conclusion3D and Z-stack CLE imaging is a unique new option for live intraoperative endomicroscopy of brain tumors. The 3D images afford an increased spatial understanding of tumor cellular architecture and visualization of related structures compared with two-dimensional images. Future application of specific fluorescent probes could benefit from this rapid in vivo imaging technology for interrogation of brain tumor tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.