ArticleScientific reports2018
Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Physical Changes of Biomacromolecules upon Covalent Surface Immobilization.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- BlinkFusion: modular platform quantifying labeling efficiency and photophysics in regular and super-resolution fluorescence microscopy.Frontiers in bioinformatics · 2026Article
- Automated morphological classification and quantification of cerebrospinal fluid extracellular vesiclesNanoscale advances · 2025Article
- Molecular landscape of the fungal plasma membrane and implications for antifungal action.Nature communications · 2025Article
- 3D bioprinting of a perfusable skin-on-chip model suitable for drug testing and wound healing studies.Materials today. Bio · 2025Article
- Freeze-cast SiOC ceramics supporting the growth of industrially relevant microorganisms.PloS one · 2025Article
- Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles.ACS omega · 2024Article
- The extracellular vesicle proteomes of Sorghum bicolor and Arabidopsis thaliana are partially conserved.Plant physiology · 2024Article
- Immobilised-enzyme microreactors for the identification and synthesis of conjugated drug metabolites.RSC advances · 2023Article
- Local extensional flows promote long-range fiber alignment in 3D collagen hydrogels.Biofabrication · 2022Article
- Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity.Materials (Basel, Switzerland) · 2022Article
- Ultra-fast and recyclable DNA biosensor for point-of-care detection of SARS-CoV-2 (COVID-19).Biosensors & bioelectronics · 2021Article
- A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes.Chembiochem : a European journal of chemical biology · 2020Article
- Combining near-infrared fluorescence with Brainbow to visualize expression of specific genes within a multicolor context.Molecular biology of the cell · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize cells without compromising their growth. Our method of immobilization is compatible with Saccharomyces cerevisiae, Escherichia coli, and synthetic cells (here, giant-unilamellar vesicles). The method introduces minimal background fluorescence and is suitable for imaging of single particles at high resolution. With S. cerevisiae we benchmarked the method against the commonly used concanavalin A approach. We show by total internal reflection fluorescence microscopy that modifying surfaces with ConA introduces artifacts close to the glass surface, which are not present when immobilizing with the APTES-glutaraldehyde method. We demonstrate validity of the method by measuring the diffusion of membrane proteins in yeast with single-particle tracking and of lipids in giant-unilamellar vesicles with fluorescence recovery after photobleaching. Importantly, the physical properties and shape of the fragile GUVs are not affected upon binding to APTES-glutaraldehyde coated glass. The APTES-glutaraldehyde is a generic method of immobilization that should work with any cell or synthetic system that has primary amines on the surface.
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