Evidence map›Paper›PMID 30213125›Full record

ArticleMolecules (Basel, Switzerland)2018

Validation of Internal Control Genes for Quantitative Real-Time PCR Gene Expression Analysis in

Qianqian Zhang, Wei Liu, Yingli Cai, A-Feng Lan, Yinbing Bian

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Qianqian ZhangInstitute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan 430070, Hubei, China. qzhang196@163.com.
Wei LiuInstitute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan 430070, Hubei, China. zhenpingliuwei@163.com.ORCID 0000-0001-7586-7117
Yingli CaiInstitute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan 430070, Hubei, China. loveylcai@163.com.
A-Feng LanSchool of Biological Science and Engineering, Shanxi University of Technology, Hanzhong 723001, Shaanxi, China. lanafeng2004@126.com.
Yinbing BianInstitute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan 430070, Hubei, China. bianyinbing@mail.hzau.edu.cn.
Huazhong Agricultural University · CNShanxi University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The reliability of qRT-PCR results depend on the stability of reference genes used for normalization, suggesting the necessity of identification of reference genes before gene expression analysis. Morels are edible mushrooms well-known across the world and highly prized by many culinary kitchens. Here, several candidate genes were selected and designed according to the

Indexed as

14-3-3 ProteinsActinsCyclinsFungal ProteinsGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Expression Regulation, FungalGenes, EssentialReal-Time Polymerase Chain ReactionReference StandardsReproducibility of ResultsSaccharomycetales14-3-3 ProteinsActinsCyclinsFungal Proteinsgene expression analysishousekeeping genemorelnormalizer genesqRT-PCR

Identifiers

PMID30213125
PMCPMC6225436
OpenAlexW2890890799

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.