ArticleNucleic acids research2018
Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Archaeal G-quadruplexes: a novel model for understanding unusual DNA/RNA structures across the tree of life.Nucleic acids research · 2026Article
- Article
- On the origins and variation of nucleotide skews of archaeal genomes.Frontiers in microbiology · 2026Article
- Review
- STORM Super-Resolution Visualization of Self-Assembled γPFD Chaperone Ultrastructures inNano letters · 2024Article
- A vector system for single and tandem expression of cloned genes and multi-colour fluorescent tagging inMicrobiology (Reading, England) · 2024Article
- Community Structure and Predicted Functions of Actively Growing Bacteria Responsive to Released Coral Mucus in Surrounding Seawater.Microbes and environments · 2023Article
- Non-equivalent genomes in polyploid prokaryotes.Nature microbiology · 2022Article
- Progress and Challenges in Archaeal Cell Biology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- BrdU Incorporation and Labeling of Nascent DNA to Investigate Archaeal Replication Using Super-Resolution Imaging.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021Review
- Single-Stranded DNA-Binding Proteins in the Archaea.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Review
- Archaeal DNA Repair Mechanisms.Biomolecules · 2020Review
- Live Imaging of a Hyperthermophilic Archaeon Reveals Distinct Roles for Two ESCRT-III Homologs in Ensuring a Robust and Symmetric Division.Current biology : CB · 2020Article
- Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the ArchaeonFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the haloarchaeon Haloferax volcanii as a model, we developed nascent DNA labeling and the functional GFP-labeled single-stranded binding protein RPA2 as novel tools to gain new insight into DNA replication and repair in live haloarchaeal cells. Our quantitative fluorescence microscopy data revealed that RPA2 forms distinct replication structures that dynamically responded to replication stress and DNA damaging agents. The number of the RPA2 foci per cell followed a probabilistic Poisson distribution, implying hitherto unnoticed stochastic cell-to-cell variation in haloarchaeal DNA replication and repair processes. The size range of haloarchaeal replication structures is very similar to those observed earlier in eukaryotic cells. The improved lateral resolution of 3D-SIM fluorescence microscopy allowed proposing that inhibition of DNA synthesis results in localized replication foci clustering and facilitated observation of RPA2 complexes brought about by chemical agents creating DNA double-strand breaks. Altogether our in vivo observations are compatible with earlier in vitro studies on archaeal single-stranded DNA binding proteins. Our work thus underlines the great potential of live cell imaging for unraveling the dynamic nature of transient molecular interactions that underpin fundamental molecular processes in the Third domain of life.
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