ArticlePloS one2018
Great phenotypic and genetic variation among successive chronic Pseudomonas aeruginosa from a cystic fibrosis patient.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Differential Metabolite Production Underlies Disruption of the Cystic Fibrosis Airway Microbiota by Pathogens.bioRxiv : the preprint server for biology · 2026Article
- Pseudomonas aeruginosa adaptation and persistence in the aspergilloma microbiome revealed by integrated multi-omics.G3 (Bethesda, Md.) · 2026Article
- Anti-pyocyanin Antibody Exhibits Cytotoxicity Protective Effects on Macrophages: A Promising Innovative Therapeutic Approach forACS pharmacology & translational science · 2025Article
- Identification and antimicrobial resistance profiling of Pseudomonas aeruginosa using multi-excitation Raman spectroscopy and computational analytics.npj antimicrobials and resistance · 2025Article
- Effect of Tobramycin on the Biofilm-Forming Forms of Pseudomonas aeruginosa Isolates Isolated from Patients with Cystic Fibrosis.Bulletin of experimental biology and medicine · 2025Article
- Tobramycin-resistant small colony variant mutant ofVirulence · 2024Article
- Phenotypic heterogeneity unveils a negative correlation between antibiotic resistance and quorum sensing inFrontiers in microbiology · 2024Article
- Spread ofAntibiotics (Basel, Switzerland) · 2023Article
- Potential quorum-sensing inhibitor ofMicrobiology spectrum · 2023Article
- Higher levels of Pseudomonas aeruginosa LasB elastase expression are associated with early-stage infection in cystic fibrosis patients.Scientific reports · 2023Article
- Article
- Pseudomonas aeruginosa Strains from Both Clinical and Environmental Origins Readily Adopt a Stable Small-Colony-Variant Phenotype Resulting from Single Mutations in c-di-GMP Pathways.Journal of bacteriology · 2022Article
- Resistance Is Not Futile: The Role of Quorum Sensing Plasticity inMicroorganisms · 2022Review
- Nonmotile Subpopulations ofJournal of bacteriology · 2022Article
- Bacterial evolution during human infection: Adapt and live or adapt and die.PLoS pathogens · 2021Review
- Pyranoanthocyanins Interfering with the Quorum Sensing ofInternational journal of molecular sciences · 2021Article
- Diagnosis and Stratification ofFrontiers in cellular and infection microbiology · 2021Article
- Phenotypic and Genomic Variability of Serial Peri-Lung TransplantationFrontiers in microbiology · 2021Article
- Antimicrobial Susceptibility Testing inAntibiotics (Basel, Switzerland) · 2020Article
- CFTR-PTEN-dependent mitochondrial metabolic dysfunction promotesScience translational medicine · 2019Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
objectivesDifferent adapted Pseudomonas aeruginosa morphotypes are found during chronic infections. Relevant biological determinants in P. aeruginosa successively isolated from a cystic fibrosis (CF) patient were analyzed in this work to gain insight into P. aeruginosa heterogeneity during chronic infection.
methodsSeventeen P. aeruginosa isolates collected from a patient over a 3 year period were included, 5 small colony variants (SCV) and 12 mucoids. The following analyses were performed: Pulsed-Field-Gel-Electrophoresis (PFGE)/Multilocus- sequence-typing (MLST)/serotype, antimicrobial susceptibility, growth curves, capacity to form biofilm, pigment production, elastase activity, motility; presence/expression of virulence/quorum sensing genes, and identification of resistance mechanisms.
resultsAll isolates had closely related PFGE patterns and belonged to ST412. Important phenotypic and genotypic differences were found. SCVs were more resistant to antimicrobials than mucoid isolates. AmpC hyperproduction and efflux pump activity were detected. Seven isolates contained two integrons and nine isolates only one integron. All SCVs showed the same OprD profile, while three different profiles were identified among mucoids. No amino acid changes were found in MutL and MutS. All isolates were slow-growing, generally produced high biofilm, had reduced their toxin expression and their quorum sensing, and showed low motility. Nevertheless, statistically significant differences were found among SCV and mucoid isolates. SCVs grew faster, presented higher biofilm formation and flicA expression; but produced less pyorubin and pyocyanin, showed lower elastase activity and rhlR, algD, and lasB expression than mucoid isolates.
conclusionThese results help to understand the molecular behavior of chronic P. aeruginosa isolates in CF patients.
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