ArticlePLoS pathogens2018
Plant A20/AN1 protein serves as the important hub to mediate antiviral immunity.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- An RGLG E3 Ubiquitin Ligase CaSIRE1 Negatively Regulates ABA-Dependent Drought Tolerance by Destabilizing CaSAP14 in Pepper.Plant, cell & environment · 2026Article
- Comparative genomic and evolutionary analysis of stress-associated proteins inFrontiers in genetics · 2026Article
- TheLife (Basel, Switzerland) · 2025Article
- Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members inPlants (Basel, Switzerland) · 2025Article
- Activators of the 26S proteasome when protein degradation increases.Experimental & molecular medicine · 2025Review
- Comprehensive Identification and Functional Analysis of Stress-Associated Protein (SAP) Genes in Osmotic Stress in Maize.International journal of molecular sciences · 2022Article
- Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes inInternational journal of molecular sciences · 2022Article
- Correction: Plant A20/AN1 protein serves as the important hub to mediate antiviral immunity.PLoS pathogens · 2020Article
- Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber.Plants (Basel, Switzerland) · 2020Article
- Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella.Parasitology research · 2020Article
- Functional domain analysis of LmSAP protein reveals the crucial role of the zinc-finger A20 domain in abiotic stress tolerance.Protoplasma · 2019Article
- Cucumber mosaic virus-induced gene silencing in banana.Scientific reports · 2019Article
- Tomato SlSAP3, a member of the stress-associated protein family, is a positive regulator of immunity against Pseudomonas syringae pv. tomato DC3000.Molecular plant pathology · 2019Article
- Genomic Analysis of Stress Associated Proteins in Soybean and the Role ofFrontiers in plant science · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salicylic acid (SA) is a key phytohormone that mediates a broad spectrum of resistance against a diverse range of viruses; however, the downstream pathway of SA governed antiviral immune response remains largely to be explored. Here, we identified an orchid protein containing A20 and AN1 zinc finger domains, designated Pha13. Pha13 is up-regulated upon virus infection, and the transgenic monocot orchid and dicot Arabidopsis overexpressing orchid Pha13 conferred greater resistance to different viruses. In addition, our data showed that Arabidopsis homolog of Pha13, AtSAP5, is also involved in virus resistance. Pha13 and AtSAP5 are early induced by exogenous SA treatment, and participate in the expression of SA-mediated immune responsive genes, including the master regulator gene of plant immunity, NPR1, as well as NPR1-independent virus defense genes. SA also induced the proteasome degradation of Pha13. Functional domain analysis revealed that AN1 domain of Pha13 is involved in expression of orchid NPR1 through its AN1 domain, whereas dual A20/AN1 domains orchestrated the overall virus resistance. Subcellular localization analysis suggested that Pha13 can be found localized in the nucleus. Self-ubiquitination assay revealed that Pha13 confer E3 ligase activity, and the main E3 ligase activity was mapped to the A20 domain. Identification of Pha13 interacting proteins and substrate by yeast two-hybrid screening revealed mainly ubiquitin proteins. Further detailed biochemical analysis revealed that A20 domain of Pha13 binds to various polyubiquitin chains, suggesting that Pha13 may interact with multiple ubiquitinated proteins. Our findings revealed that Pha13 serves as an important regulatory hub in plant antiviral immunity, and uncover a delicate mode of immune regulation through the coordination of A20 and/or AN1 domains, as well as through the modulation of E3 ligase and ubiquitin chain binding activity of Pha13.
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