ArticleFrontiers in microbiology2018
Viability RT-qPCR to Distinguish Between HEV and HAV With Intact and Altered Capsids.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 44 citations in OpenAlex.
- SARS-CoV-2 air sampling: A systematic review on the methodologies for detection and infectivity.Indoor air · 2022Pooled it
- Airborne spread of infectious SARS-CoV-2: Moving forward using lessons from SARS-CoV and MERS-CoV.The Science of the total environment · 2021Pooled it
- Article
- An RTCA-based assay as an innovative approach for thermal inactivation studies of hepatitis A virus.Applied and environmental microbiology · 2026Article
- Review
- A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment.Veterinary sciences · 2023Article
- Development of a Propidium Monoazide-Based Viability Quantitative PCR Assay for Red Sea Bream Iridovirus Detection.International journal of molecular sciences · 2023Article
- Inactivation of foodborne viruses by novel organic peroxyacid-based disinfectants.Frontiers in microbiology · 2023Article
- A viability assay combining palladium compound treatment with quantitative PCR to detect viable Mycobacterium avium subsp. paratuberculosis cells.Scientific reports · 2022Article
- Discrimination of non-infectious SARS-CoV-2 particles from fomites by viability RT-qPCR.Environmental research · 2022Article
- A triton X-100 assisted PMAxx-qPCR assay for rapid assessment of infectious African swine fever virus.Frontiers in microbiology · 2022Article
- Capsid integrity RT-qPCR for the selective detection of intact SARS-CoV-2 in wastewater.The Science of the total environment · 2021Article
- Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications - A systematic review.Water research X · 2021Article
- Detection and disinfection of COVID-19 virus in wastewater.Environmental chemistry letters · 2021Review
- A Novel Approach to the Viability Determination ofFrontiers in microbiology · 2021Article
- Hepatitis A virus survival on drug paraphernalia.Journal of viral hepatitis · 2020Article
- Key Factors for Removing Bias in Viability PCR-Based Methods: A Review.Current microbiology · 2020Review
- Rapid Selective Detection of Potentially Infectious Porcine Epidemic Diarrhea Coronavirus Exposed to Heat Treatments Using Viability RT-qPCR.Frontiers in microbiology · 2020Article
- Evaluation of High-Pressure Processing in Inactivation of the Hepatitis E Virus.Frontiers in microbiology · 2020Article
- Assessing the Occurrence of Waterborne Viruses in Reuse Systems: Analytical Limits and Needs.Pathogens (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hepatitis E virus (HEV) is an emerging pathogen showing a considerable increase in the number of reported cases in Europe mainly related to the ingestion of contaminated food. As with other relevant viral foodborne pathogens, real-time reverse transcriptase polymerase chain reaction (RT-qPCR) is the gold standard for HEV detection in clinical, food, and environmental samples, but these procedures cannot discriminate between inactivated and potentially infectious viruses. Thus, the aim of this study was to develop a viability PCR method to discriminate between native, heat-, and high-pressure processing (HPP)-treated HEV using the hepatitis A virus (HAV) as a cultivable surrogate. To this end, different concentrations of viability markers (PMAxx and platinum chloride, PtCl
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.