ArticleScientific reports2018
In vitro studies on space-conforming self-assembling silk hydrogels as a mesenchymal stem cell-support matrix suitable for minimally invasive brain application.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Dynamic Silk Fibroin Hydrogels for Programmable Bioactuation and Smart Shape Deformation: Mechanisms, Performance Evaluation, and Biomedical Applications.Gels (Basel, Switzerland) · 2026Review
- Impact of the Chronic Ischemic Stroke Microenvironment on Silk Fibroin Hydrogel Biodegradation andACS omega · 2026Article
- Silk Fibroin-Based Hydrogels Supplemented with Decellularized Extracellular Matrix and Gelatin Facilitate 3D Bioprinting for Meniscus Tissue Engineering.Macromolecular bioscience · 2025Article
- 3D biological scaffold delivers Bergenin to reduce neuroinflammation in rats with cerebral hemorrhage.Journal of translational medicine · 2024Article
- Injectable Bombyx mori (B. mori) silk fibroin/MXene conductive hydrogel for electrically stimulating neural stem cells into neurons for treating brain damage.Journal of nanobiotechnology · 2024Article
- Functionalising silk hydrogels with hetero- and homotypic nanoparticles.RSC advances · 2024Article
- Microfibre-Functionalised Silk Hydrogels.Cells · 2023Article
- Resistance to Degradation of Silk Fibroin Hydrogels Exposed to Neuroinflammatory Environments.Polymers · 2023Article
- Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering.Journal of functional biomaterials · 2022Article
- Advancements in Hydrogel Application for Ischemic Stroke Therapy.Gels (Basel, Switzerland) · 2022Review
- Impact of silk hydrogel secondary structure on hydrogel formation, silk leaching and in vitro response.Scientific reports · 2022Article
- Decellularized peripheral nerve as an injectable delivery vehicle for neural applications.Journal of biomedical materials research. Part A · 2022Article
- Decellularised extracellular matrix-based biomaterials for repair and regeneration of central nervous system.Expert reviews in molecular medicine · 2022Review
- Hydrogel Encapsulation: Taking the Therapy of Mesenchymal Stem Cells and Their Derived Secretome to the Next Level.Frontiers in bioengineering and biotechnology · 2022Review
- Silk Hydrogel Substrate Stress Relaxation Primes Mesenchymal Stem Cell Behavior in 2D.ACS applied materials & interfaces · 2021Article
- Article
- Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System.Pharmaceutics · 2021Review
- Nitinol thin films functionalized with CAR-T cells for the treatment of solid tumours.Nature biomedical engineering · 2020Article
- Neurorestoration Approach by Biomaterials in Ischemic Stroke.Frontiers in neuroscience · 2020Review
- Nanohydroxyapatite Reinforced Chitosan Composite Hydrogel with Tunable Mechanical and Biological Properties for Cartilage Regeneration.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advanced cell therapies require robust delivery materials and silk is a promising contender with a long clinical track record. Our aim was to optimise self-assembling silk hydrogels as a mesenchymal stem cell (MSC)-support matrix that would allow future minimally invasive brain application. We used sonication energy to programme the transition of silk (1-5% w/v) secondary structure from a random coil to a stable β-sheet configuration. This allowed fine tuning of self-assembling silk hydrogels to achieve space conformity in the absence of any silk hydrogel swelling and to support uniform cell distribution as well as cell viability. Embedded cells underwent significant proliferation over 14 days in vitro, with the best proliferation achieved with 2% w/v hydrogels. Embedded MSCs showed significantly better viability in vitro after injection through a 30G needle when the gels were in the pre-gelled versus post-gelled state. Silk hydrogels (4% w/v) with physical characteristics matching brain tissue were visualised in preliminary in vivo experiments to exhibit good space conformity in an ischemic cavity (intraluminal thread middle cerebral artery occlusion model) in adult male Sprague-Dawley rats (n = 3). This study informs on optimal MSC-hydrogel matrix conditions for minimally invasive application as a platform for future experiments targeting brain repair.
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Registered trials
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