ArticleBMC plant biology2018
Comparative transcriptome analysis reveals that tricarboxylic acid cycle-related genes are associated with maize CMS-C fertility restoration.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 24 citations in OpenAlex.
- Comparative transcriptome analysis provides insights into the gene regulation network of cytoplasmic male sterility in chilli pepper.AoB PLANTS · 2026Article
- Haplotypes of ATP-Binding CassetteBiomolecules · 2024Article
- Unlocking the adaptation mechanisms of the oleaginous microalgaFrontiers in microbiology · 2024Article
- Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean.Frontiers in plant science · 2023Article
- Transcriptome profiling of flower buds of male-sterile lines provides new insights into male sterility mechanism in alfalfa.BMC plant biology · 2022Article
- Identification ofInternational journal of molecular sciences · 2021Article
- Genome-wide comparative transcriptome analysis of the A4-CMS line ICPA 2043 and its maintainer ICPB 2043 during the floral bud development of pigeonpea.Functional & integrative genomics · 2021Article
- Comparative proteomic analysis of mitochondrial proteins from maize CMS-C sterile, maintainer and restorer anthers.The plant genome · 2020Article
- Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Identification and characterization of the TCA cycle genes in maize.BMC plant biology · 2019Article
- Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (Plants (Basel, Switzerland) · 2019Article
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10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundC-type cytoplasmic male sterility (CMS-C) is one of the three major types of cytoplasmic male sterility (CMS) in maize. Rf4 is a dominant restorer gene for CMS-C and has great value in hybrid maize breeding, but little information concerning its functional mechanism is known.
resultsTo reveal the functional mechanism of Rf4, we developed a pair of maize near-isogenic lines (NILs) for the Rf4 locus, which included a NIL_rf4 male-sterile line and a NIL_Rf4 male fertility-restored line. Genetic analysis and molecular marker detection indicated that the male fertility of NIL_Rf4 was controlled by Rf4. Whole-genome sequencing demonstrated genomic differences between the two NILs was clustered in the Rf4 mapping region. Unmapped reads of NILs were further assembled to uncover Rf4 candidates. RNA-Seq was then performed for the developing anthers of the NILs to identify critical genes and pathways associated with fertility restoration. A total of 7125 differentially expressed genes (DEGs) were identified. These DEGs were significantly enriched in 242 Gene Ontology (GO) categories, wherein 100 DEGs were involved in pollen tube development, pollen tube growth, pollen development, and gametophyte development. Homology analysis revealed 198 male fertility-related DEGs, and pathway enrichment analysis revealed that 58 DEGs were enriched in cell energy metabolism processes involved in glycolysis, the pentose phosphate pathway, and pyruvate metabolism. By querying the Plant Reactome Pathway database, we found that 14 of the DEGs were involved in the mitochondrial tricarboxylic acid (TCA) cycle and that most of them belonged to the isocitrate dehydrogenase (IDH) and oxoglutarate dehydrogenase (OGDH) enzyme complexes. Transcriptome sequencing and real-time quantitative PCR (qPCR) showed that all the above TCA cycle-related genes were up-regulated in NIL_Rf4. The results of our subsequent enzyme-linked immunosorbent assay (ELISA) experiments pointed out that the contents of both the IDH and OGDH enzymes accumulated more in the spikelets of NIL_Rf4 than in those of NIL_rf4.
conclusionThe present research provides valuable genomic resources for deep insight into the molecular mechanism underlying CMS-C male fertility restoration. Importantly, our results indicated that genes involved in energy metabolism, especially some mitochondrial TCA cycle-related genes, were associated with maize CMS-C male fertility restoration.
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