ArticleAsian-Australasian journal of animal sciences2019
Effects of exercise on myokine gene expression in horse skeletal muscles.
Article in Asian-Australasian journal of animal sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Transcriptomic signatures reveal systemic adaptations and immune modulation in response to training and competitive racing in horses.Equine veterinary journal · 2026Article
- Condition optimization for electroporation transfection in horse skeletal muscle satellite cells.Animal biotechnology · 2024Article
- Immunology of Physical Exercise: IsInternational journal of molecular sciences · 2024Review
- Concentrations of Selected Adipocytokines in the Blood Plasma in Proximal Suspensory Desmopathy of Horses, with a Focus on Their Physical Activity-A Pilot Study.International journal of molecular sciences · 2023Article
- Article
- Regulation of toll-like receptors expression in muscle cells by exercise-induced stress.Animal bioscience · 2021Article
- Validation of exercise-response genes in skeletal muscle cells of Thoroughbred racing horses.Animal bioscience · 2021Article
- Comparison for immunophysiological responses of Jeju and Thoroughbred horses after exercise.Asian-Australasian journal of animal sciences · 2020Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveTo examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide.
methodsThe mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells.
resultsIL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide (H2O2)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue.
conclusionWe confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.
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