ArticleScientific reports2018
Fluorescently Labelled Silica Coated Gold Nanoparticles as Fiducial Markers for Correlative Light and Electron Microscopy.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Article
- Bimodal endocytic probe for three-dimensional correlative light and electron microscopy.Cell reports methods · 2022Article
- Recent Developments in Correlative Super-Resolution Fluorescence Microscopy and Electron Microscopy.Molecules and cells · 2022Review
- Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.Frontiers in cell and developmental biology · 2022Article
- Diatom Mediated Production of Fluorescent Flower Shaped Silver-Silica Nanohybrid.Materials (Basel, Switzerland) · 2021Article
- CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.Journal of structural biology · 2021Article
- Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures.Nature protocols · 2021Article
- Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy.Journal of structural biology: X · 2021Article
- Photonic-chip assisted correlative light and electron microscopy.Communications biology · 2020Article
- An Alternative Perfusion Approach for the Intensification of Virus-Like Particle Production in HEK293 Cultures.Frontiers in bioengineering and biotechnology · 2020Article
- Development & Characterization of Fluorescently Tagged Nanocellulose for Nanotoxicological Studies.Environmental science. Nano · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, gold nanoparticles coated with a fluorescently labelled (rhodamine B) silica shell are presented as fiducial markers for correlative light and electron microscopy (CLEM). The synthesis of the particles is optimized to obtain homogeneous, spherical core-shell particles of arbitrary size. Next, particles labelled with different fluorophore densities are characterized to determine under which conditions bright and (photo)stable particles can be obtained. 2 and 3D CLEM examples are presented where optimized particles are used for correlation. In the 2D example, fiducials are added to a cryosection of cells whereas in the 3D example cells are imaged after endocytosis of the fiducials. Both examples demonstrate that the particles are clearly visible in both modalities and can be used for correlation. Additionally, the recognizable core-shell structure of the fiducials proves to be very powerful in electron microscopy: it makes it possible to irrefutably identify the particles and makes it easy to accurately determine the center of the fiducials.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.