ArticleACS chemical biology2018
Molecular Basis for Autocatalytic Backbone N-Methylation in RiPP Natural Product Biosynthesis.
Article in ACS chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Tangled Tail of Mechanically Interlocked Peptides.Journal of the American Chemical Society · 2026Review
- Characterizing and engineering post-translational modifications with high-throughput cell-free expression.Nature communications · 2025Article
- Substrate Specificity of a Methyltransferase Involved in the Biosynthesis of the Lantibiotic Cacaoidin.Biochemistry · 2024Article
- Genome Mining and Biological Engineering of Type III Borosins from Bacteria.International journal of molecular sciences · 2024Article
- Discovery of Borosin Catalytic Strategies and Function through Bioinformatic Profiling.ACS chemical biology · 2024Article
- An intramolecular macrocyclase in plant ribosomal peptide biosynthesis.Nature chemical biology · 2024Article
- Disordered regions in proteusin peptides guide post-translational modification by a flavin-dependent RiPP brominase.Nature communications · 2024Article
- A Permissive AmideACS catalysis · 2023Article
- Bioconjugate Platform for Iterative BackboneACS catalysis · 2022Article
- Structure and mechanism for iterative amideProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Molecular mechanism underlying substrate recognition of the peptide macrocyclase PsnB.Nature chemical biology · 2021Article
- Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis.Nature communications · 2021Article
- Engineering of a Peptide α-N-Methyltransferase to Methylate Non-Proteinogenic Amino Acids.Angewandte Chemie (International ed. in English) · 2021Article
- Engineering of a Peptide α-N-Methyltransferase to Methylate Non-Proteinogenic Amino Acids.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Article
- Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products.Current opinion in biotechnology · 2021Review
- Identification, heterologous production and bioactivity of lentinulin A and dendrothelin A, two natural variants of backbone N-methylated peptide macrocycle omphalotin A.Scientific reports · 2021Article
- New developments in RiPP discovery, enzymology and engineering.Natural product reports · 2021Review
- Substrate Plasticity of a Fungal Peptide α-ACS chemical biology · 2020Article
- Recent Advances in the Discovery and Biosynthetic Study of Eukaryotic RiPP Natural Products.Molecules (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
N-methylation of nucleic acids, proteins, and peptides is a chemical modification with significant impact on biological regulation. Despite the simplicity of the structural change, N-methylation can influence diverse functions including epigenetics, protein complex formation, and microtubule stability. While there are limited examples of N-methylation of the α-amino group of bacterial and eukaryotic proteins, there are no examples of catalysts that carry out post-translation methylation of backbone amides in proteins or peptides. Recent studies have identified enzymes that catalyze backbone N-methylation on a peptide substrate, a reaction with little biochemical precedent, in a family of ribosomally synthesized natural products produced in basidiomycetes. Here, we describe the crystal structures of Dendrothele bispora dbOphMA, a methyltransferase that catalyzes multiple N-methylations on the peptide backbone. We further carry out biochemical studies of this catalyst to determine the molecular details that promote this unusual chemical transformation. The structural and biochemical framework described here could facilitate biotechnological applications of catalysts for the rapid production of backbone N-methylated peptides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.