ArticleACS applied materials & interfaces2018
Protein Nanofibril Assemblies Templated by Graphene Oxide Nanosheets Accelerate Early Cell Adhesion and Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells.
Article in ACS applied materials & interfaces, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 49 citations in OpenAlex.
- Engineered protein nanofibrils for chain-integrated water treatment: From source monitoring to end-point purification.Eco-Environment & Health · 2026Review
- Recent Advances in the Design and Structural/Functional Regulations of Biomolecule-Reinforced Graphene Materials for Bone Tissue Engineering Applications.Small science · 2025Review
- Stem Cells and Bone Tissue Engineering.Life (Basel, Switzerland) · 2024Review
- Graphene Oxide-Coated Patterned Silk Fibroin Films Promote Cell Adhesion and Induce Cardiomyogenic Differentiation of Human Mesenchymal Stem Cells.Biomolecules · 2023Article
- Sustained delivery of osteogenic growth peptide through injectable photoinitiated composite hydrogel for osteogenesis.Frontiers in bioengineering and biotechnology · 2023Article
- Advancements and Applications in the Composites of Silk Fibroin and Graphene-Based Materials.Polymers · 2022Review
- Silk fibroin scaffolds: A promising candidate for bone regeneration.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- A biocompatible vascularized graphene oxide (GO)-collagen chamber with osteoinductive and anti-fibrosis effects promotes bone regenerationTheranostics · 2020Article
- Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect.Molecules (Basel, Switzerland) · 2019Article
- Stimuli-Responsive Graphene Nanohybrids for Biomedical Applications.Stem cells international · 2019Review
- Engineering Functional Graphenic Materials for Bone Repair.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Bombyx mori silk fibroin (SF) is a promising natural biocompatible protein. However, its interaction with graphene oxide (GO) has never been studied and the resultant SF/GO matrix has not been used to direct stem cell fate. Herein, we found out that mixing SF molecules and GO nanosheets in an aqueous solution can trigger the assembly of SF nanoparticles into oriented nanofibrils due to the guidance of GO nanosheets, forming SF/GO films with unique nanotopographies and improved modulus upon the removal of the solvent. When GO mass percentage in the SF/GO films is 2 and 10%, the SF assemblies are necklace-like nanofibrils (assembled from loosely linked SF nanoparticles) and solid nanofibrils (assembled from densely linked SF nanoparticles) in the resultant films, termed SG2 and SG10, respectively. GO nanosheets guided the SF assembly into nanofibrils by triggering the structural change of SF molecules from random coils to β-sheets, as confirmed by Fourier transform infrared spectroscopy and circular dichroism measurements. Furthermore, oxidative groups in the GO nanosheets were reduced by the reducing groups in SF during the nanofibril formation according to X-ray photoelectron spectroscopy and Raman spectroscopy. The reduction of the oxidative groups in GO by SF was further verified by the good cell viability on the SF/GO films. The unique nanotopographies of the SF/GO films were found to accelerate the early cell adhesion and induce the osteogenic differentiation of human mesenchymal stem cells (MSCs) even in the absence of additional inducers in the medium. More importantly, SG10 presents a stronger capability in promoting early MSC adhesion by promoting F-actin assembly, increasing cell spreading area, and inducing the osteogenic differentiation of the MSCs by the unique SF/GO nanofibrous matrix. To the best of our knowledge, it is the first report that the SF/GO substrates can induce the osteogenic differentiation of MSCs in the absence of osteogenic differentiation medium. Therefore, SF/GO composite materials would have a potential application in the field of bone tissue engineering.
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