Evidence map›Paper›PMID 30204302›Full record

ArticleCurrent protocols in molecular biology2018

RNAi Screening: Automated High-Throughput Liquid RNAi Screening in Caenorhabditis elegans.

Sakthimala Jagadeesan, Abdul Hakkim

Open access · greenAbstract read
In one paragraph

Article in Current protocols in molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Sakthimala JagadeesanDepartment of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts.
Abdul HakkimDepartment of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts.
Massachusetts General Hospital · US

Funding

Technical Mother Machine CoreP01AI083214 · NIAID · SCHEPENS EYE RESEARCH INSTITUTE · PI David C Hooper · 2009 to 2026
$39.4M
NIAID NIH HHS P01 AI083214
6 · The paper itself

Abstract

RNAi is a powerful reverse genetics tool that has revolutionized genetic studies in model organisms. The bacteriovorous nematode Caenorhabditis elegans can be genetically manipulated by feeding it an Escherichia coli strain that expresses a double-stranded RNA (dsRNA) corresponding to a C. elegans gene, which leads to systemic silencing of the gene. This unit describes protocols for performing an automated high-throughput RNAi screen utilizing a full-genome C. elegans RNAi library. The protocols employ liquid-handling robotics and 96-well plates. © 2018 by John Wiley & Sons, Inc.

Indexed as

RNA, Double-StrandedRNA InterferenceAnimalsCaenorhabditis elegansEscherichia coliHigh-Throughput Screening AssaysRNA, Double-Strandedautomated high-throughput screeningC. elegansreverse geneticsRNAiRNAi feeding

Identifiers

PMID30204302
PMCPMC6168420
OpenAlexW2889712306

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.