Evidence map›Paper›PMID 30203521›Full record

ArticleMolecular ecology resources2019

Finding Nemo's Genes: A chromosome-scale reference assembly of the genome of the orange clownfish Amphiprion percula.

Robert Lehmann, Damien J Lightfoot, Celia Schunter, Craig T Michell, Hajime Ohyanagi, Katsuhiko Mineta, Sylvain Foret, Michael L Berumen, David J Miller, Manuel Aranda and 3 more

Open access · hybridAbstract read
In one paragraph

Article in Molecular ecology resources, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 65 citations in OpenAlex.

  1. Article
  2. Host use drives convergent evolution in clownfish.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  3. Article
  4. The genome of the sapphire damselfishGigaByte (Hong Kong, China) · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. A New Zebrafish Model for Pseudoxanthoma Elasticum.Frontiers in cell and developmental biology · 2021
    Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Robert LehmannKAUST Environmental Epigenetic Program, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0001-7071-4226
Damien J LightfootKAUST Environmental Epigenetic Program, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0003-3824-8856
Celia SchunterKAUST Environmental Epigenetic Program, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0003-3620-2731
Craig T MichellRed Sea Research Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Hajime OhyanagiComputational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Katsuhiko MinetaComputational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0002-4727-045X
Sylvain ForetARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia.
Michael L BerumenRed Sea Research Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0003-2463-2742
David J MillerARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia.
Manuel ArandaRed Sea Research Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0001-6673-016X
Takashi GojoboriComputational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0001-7850-1743
Philip L MundayARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia.ORCID http://orcid.org/0000-0001-9725-2498
Timothy RavasiKAUST Environmental Epigenetic Program, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0002-9950-465X
King Abdullah University of Science and Technology · SAARC Centre of Excellence for Coral Reef Studies · AUAustralian National University · AU

Funding

King Abdullah University of Science and Technology OCRF-2014-CRG3-62140408
6 · The paper itself

Abstract

The iconic orange clownfish, Amphiprion percula, is a model organism for studying the ecology and evolution of reef fishes, including patterns of population connectivity, sex change, social organization, habitat selection and adaptation to climate change. Notably, the orange clownfish is the only reef fish for which a complete larval dispersal kernel has been established and was the first fish species for which it was demonstrated that antipredator responses of reef fishes could be impaired by ocean acidification. Despite its importance, molecular resources for this species remain scarce and until now it lacked a reference genome assembly. Here, we present a de novo chromosome-scale assembly of the genome of the orange clownfish Amphiprion percula. We utilized single-molecule real-time sequencing technology from Pacific Biosciences to produce an initial polished assembly comprised of 1,414 contigs, with a contig N50 length of 1.86 Mb. Using Hi-C-based chromatin contact maps, 98% of the genome assembly were placed into 24 chromosomes, resulting in a final assembly of 908.8 Mb in length with contig and scaffold N50s of 3.12 and 38.4 Mb, respectively. This makes it one of the most contiguous and complete fish genome assemblies currently available. The genome was annotated with 26,597 protein-coding genes and contains 96% of the core set of conserved actinopterygian orthologs. The availability of this reference genome assembly as a community resource will further strengthen the role of the orange clownfish as a model species for research on the ecology and evolution of reef fishes.

Indexed as

ChromosomesGene OrderAnimalsComputational BiologyFishesGenomicsPacific OceanSequence Analysis, DNAAmphiprion perculachromosome-scale assemblycoral reef fishfish genomicsfunctional genomicsNemoorange clownfish

Identifiers

PMID30203521
PMCPMC7379943
OpenAlexW2949894468

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.