ArticleRice (New York, N.Y.)2018
The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively.
Article in Rice (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- A cellular view of seed development and germination.The Plant cell · 2026Review
- Genome-wide identification and expression profiling of the bHLH transcription factor family under salt stress and hormonal treatments in cowpea (Vigna unguiculata).Scientific reports · 2026Article
- Transcriptomic Profiling Reveals the Seed Aging Process inPlants (Basel, Switzerland) · 2026Article
- Functional Analysis ofPlants (Basel, Switzerland) · 2026Article
- Article
- bHLH Transcription Factors in Cereal Crops: Diverse Functions in Regulating Growth, Development and Stress Responses.International journal of molecular sciences · 2025Review
- Genome-wide association studies reveal genetic diversity and regulatory loci underlying dwarfing traits in banana.Journal of integrative plant biology · 2025Article
- Multi-omics-Based Construction of ncRNA-Gene-Metabolite Networks Provides New Insights Into Metabolic Regulation Under Salt Stress in Rice.Rice (New York, N.Y.) · 2025Article
- Genome-wide analysis of the Tritipyrum bHLH gene family and the response of TtbHLH310 in salt-tolerance.BMC genomics · 2025Article
- Comparative Transcriptome Analysis Reveals Mechanisms of Differential Salinity Tolerance BetweenGenes · 2024Article
- Physiological and transcriptomic analysis reveals the coating of microcapsules embedded with bacteria can enhance wheat salt tolerance.BMC plant biology · 2024Article
- Accurate Long-Read RNA Sequencing Analysis Reveals the Key Pathways and Candidate Genes under Drought Stress in the Seed Germination Stage in Faba Bean.International journal of molecular sciences · 2024Article
- Deciphering molecular regulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) signalling networks in Oryza genus amid environmental stress.Plant cell reports · 2024Review
- Variation in TaSPL6-D confers salinity tolerance in bread wheat by activating TaHKT1;5-D while preserving yield-related traits.Nature genetics · 2024Article
- Uncovering Novel QTLs and Candidate Genes for Salt Tolerance at the Bud Burst Stage in Rice through Genome-Wide Association Study.Plants (Basel, Switzerland) · 2024Article
- bHLH-regulated routes in anther development in rice and Arabidopsis.Genetics and molecular biology · 2024Article
- Genome expression analysis of basic helix-loop-helix transcription factors in Sea buckthorn (Frontiers in plant science · 2024Article
- Dynamic physiological and transcriptomic changes reveal memory effects of salt stress in maize.BMC genomics · 2023Article
- Sugar starvation activates the OsSnRK1a-OsbHLH111/OsSGI1-OsTPP7 module to mediate growth inhibition of rice.Plant biotechnology journal · 2023Article
- Integrated Analysis of Transcriptome and Metabolome Reveals Molecular Mechanisms of Rice with Different Salinity Tolerances.Plants (Basel, Switzerland) · 2023Article
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Abstract
backgroundMany transcription factors (TFs), such as those in the basic helix-loop-helix (bHLH) family, are important for regulating plant growth and plant responses to abiotic stress. The expression of OsbHLH035 is induced by drought and salinity. However, its functional role in rice growth, development, and the salt response is still unknown.
resultsThe bHLH TF OsbHLH035 is a salt-induced gene that is primarily expressed in germinating seeds and seedlings. Stable expression of GFP-fused OsbHLH035 in rice transgenic plants revealed that this protein is predominantly localized to the nucleus. Osbhlh035 mutants show delayed seed germination, particularly under salt-stress conditions. In parallel, abscisic acid (ABA) contents are over-accumulated, and the expression of the ABA biosynthetic genes OsABA2 and OsAAO3 is upregulated; furthermore, compared with that in wild-type (WT) seedlings, the salt-induced expression of OsABA8ox1, an ABA catabolic gene, in germinating Osbhlh035 mutant seeds is downregulated. Moreover, Osbhlh035 mutant seedlings are unable to recover from salt-stress treatment. Consistently, sodium is over-accumulated in aerial tissues but slightly reduced in terrestrial tissues from Osbhlh035 seedlings after salt treatment. Additionally, the expression of the sodium transporters OsHKT1;3 and 1;5 is reduced in Osbhlh035 aerial and terrestrial tissues, respectively. Furthermore, genetic complementation can restore both the delayed seed germination and the impaired recovery of salt-treated Osbhlh035 seedlings to normal growth.
conclusionOsbHLH035 mediates seed germination and seedling recovery after salt stress relief through the ABA-dependent and ABA-independent activation of OsHKT pathways, respectively.
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