Evidence map›Paper›PMID 30203285›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel.

Guolin Ma, Lian He, Ji Jing, Peng Tan, Yun Huang, Yubin Zhou

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Guolin MaCenter for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W. Holcombe Blvd, Houston, TX, USA.
Lian HeCenter for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W. Holcombe Blvd, Houston, TX, USA.
Ji JingCenter for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W. Holcombe Blvd, Houston, TX, USA.
Peng TanCenter for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W. Holcombe Blvd, Houston, TX, USA.
Yun HuangCenter for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W. Holcombe Blvd, Houston, TX, USA.
Yubin ZhouCenter for Translational Cancer Research, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W. Holcombe Blvd, Houston, TX, USA. yzhou@ibt.tamhsc.edu.
Texas A&M University · US

Funding

MOLECULAR CONTROL OF CALCIUM INFLUX AT THE ER-PLASMA MEMBRANE JUNCTIONSR01GM112003 · NIGMS · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI ZHOU, YUBIN · 2014 to 2022
$2.7M
TET-mediated epigenetic regulation in cardiac development.R01HL134780 · NHLBI · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI HUANG, YUN · 2018 to 2022
$1.9M
Optogenetic toolkit for remote control of voltage-gated calcium channelsR21GM126532 · NIGMS · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI HAN, GANG, ZHOU, YUBIN · 2018 to 2019
$443k
NHLBI NIH HHS R01 HL134780NIGMS NIH HHS R01 GM112003NIGMS NIH HHS R21 GM126532
6 · The paper itself

Abstract

ORAI1 constitutes the pore-forming subunit of the calcium release-activated calcium (CRAC) channel, a prototypical store-operated channel that is essential for the activation of cells of the immune system. Here we describe a convenient yet powerful cross-linking approach to examine the pore architecture of CRAC channels using ORAI1 proteins engineered to contain one or two cysteine residues. The generalizable cross-linking in situ approach can also be readily extended to study other integral membrane proteins expressed in various types of cells.

Indexed as

CalciumCalcium Release Activated Calcium ChannelsCalcium SignalingCloning, MolecularCysteineGene ExpressionGenetic EngineeringHEK293 CellsHumansIon Channel GatingMutagenesis, Site-DirectedPlasmidsProtein MultimerizationStructure-Activity RelationshipCalciumCalcium Release Activated Calcium ChannelsCysteineCalcium release-activated calcium channelCalcium signalingCross-linkingDisulfide bondLymphocyte activationMembrane proteinORAI1Store-operated calcium entryStromal interaction moleculeStructure

Identifiers

PMID30203285
PMCPMC8935632
OpenAlexW2890638500

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.