Evidence map›Paper›PMID 30202095›Full record

ArticleScientific reports2018

Bioengineering functional smooth muscle with spontaneous rhythmic contraction in vitro.

Masae Kobayashi, Hassan A Khalil, Nan Ye Lei, Qianqian Wang, Ke Wang, Benjamin M Wu, James C Y Dunn

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Bioengineering of Intestinal Grafts.Gastroenterology clinics of North America · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Masae KobayashiDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Hassan A KhalilDepartment of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Nan Ye LeiDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Qianqian WangDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Ke WangDepartment of Computer Science, University of North Carolina Chapel Hill, North Carolina, NC, 27514, USA.
Benjamin M WuDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
James C Y DunnDepartment of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA. jdunn2@stanford.edu.
University of California, Los Angeles · USSamueli Institute · USUniversity of North Carolina at Chapel Hill · US

Funding

NIDDK NIH HHS R01 DK083119
6 · The paper itself

Abstract

Oriented smooth muscle layers in the intestine contract rhythmically due to the action of interstitial cells of Cajal (ICC) that serve as pacemakers of the intestine. Disruption of ICC networks has been reported in various intestinal motility disorders, which limit the quality and expectancy of life. A significant challenge in intestinal smooth muscle engineering is the rapid loss of function in cultured ICC and smooth muscle cells (SMC). Here we demonstrate a novel approach to maintain the function of both ICC and SMC in vitro. Primary intestinal SMC mixtures cultured on feeder cells seeded electrospun poly(3-caprolactone) scaffolds exhibited rhythmic contractions with directionality for over 10 weeks in vitro. The simplicity of this system should allow for wide usage in research on intestinal motility disorders and tissue engineering, and may prove to be a versatile platform for generating other types of functional SMC in vitro.

Indexed as

AnimalsAnimals, NewbornCell LineColonoscopyFemaleFibroblastsGastrointestinal MotilityHumansIntestinal DiseasesIntestinesMaleMiceMice, Inbred C57BLMuscle ContractionMuscle, SmoothMyocytes, Smooth MusclepolycaprolactonePolyesters

Identifiers

PMID30202095
PMCPMC6131399
OpenAlexW2892339240

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.