ArticleNature methods2018
Three-photon imaging of mouse brain structure and function through the intact skull.
Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 142 papers.
What it found
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Who cites it
142 citing papers in PubMed, 340 citations in OpenAlex.
- Depth-adapted adaptive optics for three-photon microscopy.Biomedical optics express · 2026Article
- The extracellular matrix as a dynamic regulator of brain function and plasticity.Neurobiology of disease · 2026Review
- Simultaneous three-photon and optical coherence microscopy deep within an intact mouse brain.Npj imaging · 2026Article
- Three photon microscopy of mouse brain structure and function at 2 mm depth and beyond.bioRxiv : the preprint server for biology · 2026Article
- Choosing a skull clearing technique for chronic mesoscopic optical imaging in awake mice.Neurophotonics · 2026Article
- Targeted Covalent Photoswitch for Two-Photon Control of Endogenous Receptors.Journal of the American Chemical Society · 2026Article
- A Three-dimensional Analytical Framework for Retinal Microvasculature Reveals Layer-associated Vulnerability in Development and Neovascular Remodeling.bioRxiv : the preprint server for biology · 2026Article
- Noninvasive photoacoustic computed mesoscopy for longitudinal brain imaging.Science advances · 2026Article
- Article
- Longitudinal three-photon imaging for tracking amyloid plaques and vascular degeneration in a mouse model of Alzheimer's disease.Journal of biomedical optics · 2026Article
- Advanced Microscopy Techniques.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice.Nature communications · 2025Article
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- Imaging the brain by traversing the skull with light and sound.Nature biomedical engineering · 2025Review
- Color-resolved third harmonic generation microscopy for single-RBC HbA1c measurement and glycemic history assessment.Science advances · 2025Article
- Color-neutral and reversible tissue transparency enables longitudinal deep-tissue imaging in live mice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- SeeThrough: a rationally designed skull clearing technique for in vivo brain imaging.Nature communications · 2025Article
- Compressive Fourier-Domain Intensity Coupling (C-FOCUS) enables near-millimeter deep imaging in the intact mouse brain in vivo.Research square · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
82 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even when we used the same excitation wavelength and imaging system. Here we demonstrate three-photon imaging of vasculature through the adult mouse skull at >500-μm depth, as well as GCaMP6s calcium imaging over weeks in cortical layers 2/3 and 4 in awake mice, with 8.5 frames per second and a field of view spanning hundreds of micrometers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.