ArticleScientific reports2018
Co-overexpression of native phospholipid-biosynthetic genes plsX and plsC enhances lipid production in Synechocystis sp. PCC 6803.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 34 citations in OpenAlex.
- Synechocystis sp. PCC 6803 with ddh disruption and heterologous pk gene expression in the phosphoketolase pathway drives carbon flux toward polyhydroxybutyrate production.Biotechnology for biofuels and bioproducts · 2026Article
- Supplement C18:1 in Culture Medium to Improve Survival Rate and Fermentation Activity of Lyophilization ofJournal of microbiology and biotechnology · 2025Article
- Article
- Induced Mutagenesis and Comparative Genomics ofMicroorganisms · 2025Article
- Triple-Gene Overexpression of the AcrA-AcrB-TolC Transporter System inInternational journal of molecular sciences · 2024Article
- The adc1 knockout with proC overexpression in Synechocystis sp. PCC 6803 induces a diversion of acetyl-CoA to produce more polyhydroxybutyrate.Biotechnology for biofuels and bioproducts · 2024Article
- Improved lipid production and component of mycosporine-like amino acids by co-overexpression of amt1 and aroB genes in Synechocystis sp. PCC6803.Scientific reports · 2023Article
- Increased Biomass and Polyhydroxybutyrate Production byInternational journal of molecular sciences · 2023Article
- Incorporation, fate, and turnover of free fatty acids in cyanobacteria.FEMS microbiology reviews · 2023Review
- Highlighting the potential ofFrontiers in microbiology · 2023Article
- Enhanced productivity of extracellular free fatty acids by gene disruptions of acyl-ACP synthetase and S-layer protein in Synechocystis sp. PCC 6803.Biotechnology for biofuels and bioproducts · 2022Article
- Overexpression ofInternational journal of molecular sciences · 2021Article
- CyanobacteriumBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Photosynthetic Conversion of Carbon Dioxide to Oleochemicals by Cyanobacteria: Recent Advances and Future Perspectives.Frontiers in microbiology · 2020Review
- Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineeredBiotechnology for biofuels · 2019Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The overexpression of native plsX and plsC genes involving in fatty acid/phospholipid synthesis first timely-reported the significantly enhanced lipid contents in Synechocystis sp. PCC 6803. Growth rate, intracellular pigment contents including chlorophyll a and carotenoids, and oxygen evolution rate of all overexpressing (OX) strains were normally similar as wild type. For fatty acid compositions, saturated fatty acid, in particular palmitic acid (16:0) was dominantly increased in OX strains whereas slight increases of unsaturated fatty acids were observed, specifically linoleic acid (18:2) and alpha-linolenic acid (18:3). The plsC/plsX-overexpressing (OX + XC) strain produced high lipid content of about 24.3%w/dcw under normal condition and was further enhanced up to 39.1%w/dcw by acetate induction. This OX + XC engineered strain was capable of decreasing phaA transcript level which related to poly-3-hydroxybutyrate (PHB) synthesis under acetate treatment. Moreover, the expression level of gene transcripts revealed that the plsX- and plsC/plsX-overexpression strains had also increased accA transcript amounts which involved in the irreversible carboxylation of acetyl-CoA to malonyl-CoA. Altogether, these overexpressing strains significantly augmented higher lipid contents when compared to wild type by partly overcoming the limitation of lipid production.
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