ArticleArthritis research & therapy2018
Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state.
Article in Arthritis research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Mechanisms of monosodium urate crystal-induced bone destruction in gouty arthritis (Review).Biomedical reports · 2026Review
- Application of a Novel Ultrasound Scoring System in Assessing Disease Activity in Gouty Arthritis.Journal of inflammation research · 2026Article
- Treatment of gouty lumbar spinal stenosis: a case report and bioinformatics analysis.BMC musculoskeletal disorders · 2025Article
- Serum Uric Acid and Bone Health in Middle-Aged and Elderly Hypertensive Patients: A Potential U-Shaped Association and Implications for Future Fracture Risk.Metabolites · 2025Article
- The pathogenesis of gout.Journal of rheumatic diseases · 2025Review
- Comprehensive Treatment of Gout with Traditional Chinese Medicine: A Modern Pathophysiological Perspective.International journal of general medicine · 2025Review
- The Progress of Immune Cells-induced Inflammatory Response in Gout.Current pharmaceutical design · 2025Review
- Clinical characteristics and risk factors associated with bone erosion in patients with tophi.Advances in rheumatology (London, England) · 2024Article
- Association between asymptomatic hyperuricemia and risk of arthritis, findings from a US National Survey 2007-2018.BMJ open · 2024Article
- Imaging comparative analysis of familial and sporadic gout in Chinese men by multijoint ultrasonography.Frontiers in medicine · 2024Article
- Relationship between serum uric acid levels and osteoporosis.Endocrine connections · 2023Review
- Detailed analysis of the association between urate deposition and bone erosion in gout: a dual-energy computed tomography study.Frontiers in endocrinology · 2023Article
- Osteogenic Potential of Monosodium Urate Crystals in Synovial Mesenchymal Stem Cells.Medicina (Kaunas, Lithuania) · 2022Article
- Orthopaedic Management of Gout.Journal of the American Academy of Orthopaedic Surgeons. Global research & reviews · 2022Article
- Factors secreted by monosodium urate crystal-stimulated macrophages promote a proinflammatory state in osteoblasts: a potential indirect mechanism of bone erosion in gout.Arthritis research & therapy · 2022Article
- Lipoxin A4 attenuates MSU-crystal-induced NLRP3 inflammasome activation through suppressing Nrf2 thereby increasing TXNRD2.Frontiers in immunology · 2022Article
- Role of microRNA alternation in the pathogenesis of gouty arthritis.Frontiers in endocrinology · 2022Review
- Excess Uric Acid Induces Gouty Nephropathy Through Crystal Formation: A Review of Recent Insights.Frontiers in endocrinology · 2022Review
- The Inhibition of Osteoblast Viability by Monosodium Urate Crystal-Stimulated Neutrophil-Derived Exosomes.Frontiers in immunology · 2022Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBone erosion is a frequent complication of gout and is strongly associated with tophi, which are lesions comprising inflammatory cells surrounding collections of monosodium urate (MSU) crystals. Osteocytes are important cellular mediators of bone remodeling. The aim of this study was to investigate the direct effects of MSU crystals and indirect effects of MSU crystal-induced inflammation on osteocytes.
methodsFor direct assays, MSU crystals were added to MLO-Y4 osteocyte cell line cultures or primary mouse osteocyte cultures. For indirect assays, the RAW264.7 macrophage cell line was cultured with or without MSU crystals, and conditioned medium from these cultures was added to MLO-Y4 cells. MLO-Y4 cell viability was assessed using alamarBlue® and LIVE/DEAD® assays, and MLO-Y4 cell gene expression and protein expression were assessed by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Histological analysis was used to examine the relationship between MSU crystals, inflammatory cells, and osteocytes in human joints affected by tophaceous gout.
resultsIn direct assays, MSU crystals reduced MLO-Y4 cell and primary mouse osteocyte viability but did not alter MLO-Y4 cell gene expression. In contrast, conditioned medium from MSU crystal-stimulated RAW264.7 macrophages did not affect MLO-Y4 cell viability but significantly increased MLO-Y4 cell expression of osteocyte-related factors including E11, connexin 43, and RANKL, and inflammatory mediators such as interleukin (IL)-6, IL-11, tumor necrosis factor (TNF)-α and cyclooxygenase-2 (COX-2). Inhibition of COX-2 in MLO-Y4 cells significantly reduced the indirect effects of MSU crystals. In histological analysis, CD68
conclusionsMSU crystals directly inhibit osteocyte viability and, through interactions with macrophages, indirectly promote a shift in osteocyte function that favors bone resorption and inflammation. These interactions may contribute to disordered bone remodeling in gout.
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