Evidence map›Paper›PMID 30201010›Full record

ArticleJournal of experimental & clinical cancer research : CR2018

LncRNA SLCO4A1-AS1 facilitates growth and metastasis of colorectal cancer through β-catenin-dependent Wnt pathway.

Jiangtao Yu, Zhiyang Han, Ziquan Sun, Yue Wang, Ming Zheng, Chunfang Song

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental & clinical cancer research : CR, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed, 2 pooled it
5.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 2 syntheses or guidelines pooled it, 115 citations in OpenAlex.

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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Jiangtao YuDepartment of General Surgery, the Sanya Hongsen Hospital of Harbin Medical University, Fenghuang Road, Sanya, 572000, China.
Zhiyang HanDepartment of General Surgery, the Sanya Hongsen Hospital of Harbin Medical University, Fenghuang Road, Sanya, 572000, China.
Ziquan SunDepartment of General Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
Yue WangDepartment of Pharmacology and Toxicology, Wright State University, Fairborn, OH, 45435, USA.
Ming ZhengDepartment of Ultrasonography, the Sanya Hongsen Hospital of Harbin Medical University, Sanya, 572000, China.
Chunfang SongDepartment of General Surgery, the Sanya Hongsen Hospital of Harbin Medical University, Fenghuang Road, Sanya, 572000, China. cf_song01@163.com.
Harbin Medical University · CNFirst Affiliated Hospital of Harbin Medical University · CNSanya University · CNWright State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEmerging evidence has shown long noncoding RNAs (lncRNAs) exert important roles in colorectal cancer (CRC) tumorigenesis. However, most lncRNAs involved in this process remain undefined and the underlying molecular mechanisms mediated by lncRNAs are largely unknown.

methodsAn unbiased screening was used to identify novel lncRNAs involved in CRC according to an online-available data dataset. In situ hybridization (ISH) and qRT-PCR was used to detect lncRNA expression patterns. CCK8, colony formation, fluorescence activated cell sorter (FACS), transwell, xenograft nude mouse model and western blot assays were used to analyze the functions of SLCO4A1-AS1. RNA-pulldown, western blot, RNA fluorescence in situ hybridization (RNA-FISH) and electrophoretic mobility shift assay (EMSA) assays were utilized to explore the molecular mechanism of SLCO4A1-AS1.

resultsLncRNA SLCO4A1-AS1 was significantly upregulated in CRC tissues and its overexpression was closely related with poor prognosis and tumor metastasis. By knocking down SLCO4A1-AS1, we found that SLCO4A1-AS1 promoted the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of CRC cells in vitro, as well as inhibited cell apoptosis. Moreover, SLCO4A1-AS1 dramatically delayed tumor propagation in vivo. Mechanistically, SLCO4A1-AS1 activates Wnt/β-catenin signaling. SLCO4A1-AS1 enhanced the stability of β-catenin by impairing the interaction of β-catenin with GSKβ and inhibiting its phosphorylation. Finally, restoration of β-catenin protein level rescued the proliferation, migration and invasion in SLCO4A1-AS1-depleted CRC cells.

conclusionSLCO4A1-AS1 serves as an oncogenic role in CRC through activating Wnt/β-catenin signaling pathway. And SLCO4A1-AS1 might be a useful biomarker for CRC diagnosis and prognosis.

Indexed as

AnimalsApoptosisbeta CateninCarcinogenesisCell Line, TumorCell MovementCell ProliferationColorectal NeoplasmsEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansIn Situ Hybridization, FluorescenceMiceOrganic Anion TransportersRNA, Long NoncodingWnt Signaling Pathwaybeta CateninCTNNB1 protein, humanOrganic Anion TransportersRNA, Long NoncodingSLCO4A1 protein, humanAS1Colorectal cancerSLCO4A1Tumorigenesisβ-catenin

Identifiers

PMID30201010
PMCPMC6131861
OpenAlexW2889622422

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.