Evidence map›Paper›PMID 30200803›Full record

ArticleRenal failure2018

The protective effects of rapamycin on cell autophagy in the renal tissues of rats with diabetic nephropathy via mTOR-S6K1-LC3II signaling pathway.

Lei Liu, Lijuan Yang, Baochao Chang, Jiqiang Zhang, Yaling Guo, Xiangdong Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Renal failure, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 39 citations in OpenAlex.

  1. Review
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  11. Programmed Cell Death in Diabetic Nephropathy: A Review of Apoptosis, Autophagy, and Necroptosis.Medical science monitor : international medical journal of experimental and clinical research · 2022
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Autophagy in diabetic nephropathy: a review.International urology and nephrology · 2020
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Lei Liua Department of Nephrology , Shandong University Qilu Hospital , Jinan , P.R. China.
Lijuan Yangb Department of Physiology , Bengbu Medical College , Bengbu , P.R. China.
Baochao Changc Department of Nephrology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , P.R. China.
Jiqiang Zhangc Department of Nephrology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , P.R. China.
Yaling Guoc Department of Nephrology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , P.R. China.
Xiangdong Yanga Department of Nephrology , Shandong University Qilu Hospital , Jinan , P.R. China.
First Affiliated Hospital of Bengbu Medical College · CNQilu Hospital of Shandong University · CNBengbu Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPrevious studies have shown that podocyte autophagy is an important trigger for proteinuria and glomerulosclerosis. The mammalian rapamycin target protein (mTOR) occupies a pivotal position in the autophagy pathway. In this study, we planned to clarify the mechanism of mTOR regulation of podocyte autophagy and the effect of rapamycin (RAPA).

methodsAll rats were randomly divided into normal control group (n = 8), DN group (n = 8), and RAPA group (n = 8). Blood and urine samples were collected at the 4th, 8th, and 12th weeks of the experiment. The serum creatinine (Scr), urine volume levels, and the 24 h urine protein (UP) levels were examined. The nephrin, podocin, mTOR, ribosomal S6 kinase 1 (S6K1), and autophagy marker light chain 3 (LC3II) expression levels were evaluated by immunohistochemistry, quantitative PCR, and immunoblotting.

resultsThe urine volume, 24 h UP, and Scr of the DN and RAPA groups increased significantly compared with the NC group (p < .05). Nephrin and podocin expression was decreased in the kidney tissues of the DN and RAPA groups compared with the NC group (p < .05). The expression levels of mTOR and S6K1 increased and LC3II expression decreased in the renal tissues of the DN and RAPA groups compared with the NC group (p < .05). After RAPA treatment, all the above indexes were improved compared with the DN group (p < .05), but were significantly abnormal compared with the NC group (p < .05).

conclusionThe proteinuria and kidney function had improved after RAPA treatment. These results confirmed that RAPA specifically binds to mTOR kinase, and inhibits mTOR activity, thereby regulating the pathological autophagic process.

Indexed as

AnimalsAutophagyCreatinineDiabetes Mellitus, ExperimentalDiabetic NephropathiesIntracellular Signaling Peptides and ProteinsMaleMembrane ProteinsPodocytesProteinuriaRatsRats, Sprague-DawleyRibosomal Protein S6 KinasesSignal TransductionSirolimusStreptozocinCreatinineIntracellular Signaling Peptides and ProteinsMembrane ProteinsnephrinNPHS2 proteinRibosomal Protein S6 KinasesSirolimusStreptozocinTOR Serine-Threonine KinasesautophagyDiabetic nephropathymTORpodocyterapamycin

Identifiers

PMID30200803
PMCPMC6136383
OpenAlexW2890298129

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.