Evidence map›Paper›PMID 30199303›Full record

ArticlemAbs

Site-specific conjugation allows modulation of click reaction stoichiometry for pretargeted SPECT imaging.

Danielle Mandikian, Hanine Rafidi, Pragya Adhikari, Priya Venkatraman, Lidia Nazarova, Gabriel Fung, Isabel Figueroa, Gregory Z Ferl, Sheila Ulufatu, Jason Ho and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mAbs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Danielle Mandikiana Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.
Hanine Rafidia Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.
Pragya Adhikarib Protein Chemistry , Genentech Inc ., South San Francisco , CA , USA.
Priya Venkatramana Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.ORCID 0000-0002-6298-3167
Lidia Nazarovaa Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.ORCID 0000-0001-8010-1167
Gabriel Funga Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.ORCID 0000-0001-5047-3509
Isabel Figueroaa Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.ORCID 0000-0001-9204-9132
Gregory Z Ferla Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.
Sheila Ulufatuc In Vivo Studies , Genentech Research and Early Development , South San Francisco , CA , USA.
Jason Hoc In Vivo Studies , Genentech Research and Early Development , South San Francisco , CA , USA.
Cynthia McCaugheyc In Vivo Studies , Genentech Research and Early Development , South San Francisco , CA , USA.ORCID 0000-0002-5049-7399
Jeffrey Laud Translational Oncology , Genentech Inc ., South San Francisco , CA , USA.
Shang-Fan Yud Translational Oncology , Genentech Inc ., South San Francisco , CA , USA.
Saileta Prabhua Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.
Jack Sadowskyb Protein Chemistry , Genentech Inc ., South San Francisco , CA , USA.
C Andrew Boswella Preclinical and Translational Pharmacokinetics , Genentech Inc ., South San Francisco , CA , USA.ORCID 0000-0002-0426-9846

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibody pretargeting is a promising strategy for improving molecular imaging, wherein the separation in time of antibody targeting and radiolabeling can lead to rapid attainment of high contrast, potentially increased sensitivity, and reduced patient radiation exposure. The inverse electron demand Diels-Alder 'click' reaction between trans-cyclooctene (TCO) conjugated antibodies and radiolabeled tetrazines presents an ideal platform for pretargeted imaging due to rapid reaction kinetics, bioorthogonality, and potential for optimization of both slow and fast clearing components. Herein, we evaluated a series of anti-human epidermal growth factor receptor 2 (HER2) pretargeting antibodies containing distinct molar ratios of site-specifically incorporated TCO. The effect of stoichiometry on tissue distribution was assessed for pretargeting TCO-modified antibodies (monitored by

Indexed as

AnimalsAntibodies, MonoclonalCell Line, TumorClick ChemistryErb-b2 Receptor Tyrosine KinasesHeterocyclic Compounds, 1-RingHumansImmunoconjugatesIsotope LabelingMiceNeoplasmsRadioimmunotherapyTissue DistributionTomography, Emission-Computed, Single-PhotonXenograft Model Antitumor AssaysAntibodies, MonoclonalERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesHeterocyclic Compounds, 1-RingImmunoconjugatestetrazine dye 10biorthogonal click chemistryiEDDA reactionpretargeted imagingsite-specific bioconjugationSPECTtetrazine

Identifiers

PMID30199303
PMCPMC6284555

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.