Evidence map›Paper›PMID 30198886›Full record

ArticleActa crystallographica. Section F, Structural biology communications2018

MSMEG_6292, a Mycobacterium smegmatis RNA polymerase secondary channel-binding protein: purification, crystallization and X-ray diffraction analysis.

Abyson Joseph, Valakunja Nagaraja, Ramanathan Natesh

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Abyson JosephSchool of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum, Kerala 695 016, India.
Valakunja NagarajaMicrobiology and Cell Biology Unit, Indian Institute of Science, Bangalore, Karnataka, India.
Ramanathan NateshSchool of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum, Kerala 695 016, India.ORCID 0000-0002-1145-5962
Indian Institute of Science Education and Research Thiruvananthapuram · INIndian Institute of Science Bangalore · IN

Funding

Council of Scientific and Industrial ResearchDepartment of Biotechnology, Ministry of Science and Technology BT/HRD/35/02/19/2009Ministry of Human Resource Development
6 · The paper itself

Abstract

The transcriptional activity of RNA polymerase (RNAP) is controlled by a diverse set of regulatory factors. A subset of these regulators modulate the activity of RNAP through its secondary channel. Gre factors reactivate stalled elongation complexes by enhancing the intrinsic cleavage activity of RNAP. In the present study, the protein MSMEG_6292, a Gre-factor homologue from Mycobacterium smegmatis, was expressed heterologously in Escherichia coli and purified using standard chromatographic techniques. The hanging-drop vapour-diffusion crystallization method yielded diffraction-quality crystals. The crystals belonged to the trigonal space group P3

Indexed as

Amino Acid SequenceBacterial ProteinsCloning, MolecularCrystallizationCrystallography, X-RayDNA-Directed RNA PolymerasesEscherichia coliGene ExpressionGenetic VectorsMycobacterium smegmatisPeptide Elongation FactorsRecombinant ProteinsBacterial ProteinsDNA-Directed RNA PolymerasesPeptide Elongation FactorsRecombinant ProteinsGre-factor homologueMSMEG_6292mycobacteriaMycobacterium smegmatisRNA polymerasetranscription regulationX-ray diffraction

Identifiers

PMID30198886
PMCPMC6130422
OpenAlexW2889035527

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.