Evidence map›Paper›PMID 30198885›Full record

ArticleActa crystallographica. Section F, Structural biology communications2018

TssA from Burkholderia cenocepacia: expression, purification, crystallization and crystallographic analysis.

Hayley J Owen, Ruyue Sun, Asma Ahmad, Svetlana E Sedelnikova, Patrick J Baker, Mark S Thomas, David W Rice

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hayley J OwenDepartment of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Western Bank, Sheffield S10 2TN, England.
Ruyue SunDepartment of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, England.
Asma AhmadDepartment of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, England.
Svetlana E SedelnikovaDepartment of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Western Bank, Sheffield S10 2TN, England.
Patrick J BakerDepartment of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Western Bank, Sheffield S10 2TN, England.ORCID 0000-0003-1995-5643
Mark S ThomasDepartment of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, England.ORCID 0000-0003-0701-2584
David W RiceDepartment of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Western Bank, Sheffield S10 2TN, England.ORCID 0000-0002-7811-0539

Funding

BBSRC BB/J014443/1
6 · The paper itself

Abstract

TssA is a core component of the type VI secretion system, and phylogenetic analysis of TssA subunits from different species has suggested that these proteins fall into three distinct clades. Whilst representatives of two clades, TssA1 and TssA2, have been the subjects of investigation, no members of the third clade (TssA3) have been studied. Constructs of TssA from Burkholderia cenocepacia, a representative of clade 3, were expressed, purified and subjected to crystallization trials. Data were collected from crystals of constructs of the N-terminal and C-terminal domains. Analysis of the data from the crystals of these constructs and preliminary structure determination indicates that the C-terminal domain forms an assembly of 32 subunits in D

Indexed as

ElectronsAmino Acid SequenceBacterial ProteinsBurkholderia cenocepaciaCloning, MolecularCrystallizationCrystallography, X-RayEscherichia coliGene ExpressionGenetic VectorsMembrane ProteinsPhylogenyProtein Conformation, alpha-HelicalProtein DomainsProtein SubunitsRecombinant ProteinsBacterial ProteinsMembrane ProteinsProtein SubunitsRecombinant ProteinsType VI Secretion SystemsBurkholderia cenocepaciaTssAtype VI secretion system

Identifiers

PMID30198885
PMCPMC6130427

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.