Evidence map›Paper›PMID 30198622›Full record

ArticleProtein science : a publication of the Protein Society2018

A threonine zipper that mediates protein-protein interactions: Structure and prediction.

Curran Oi, John D Treado, Zachary A Levine, Christopher S Lim, Kirsten M Knecht, Yong Xiong, Corey S O'Hern, Lynne Regan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Red fluorescent proteins engineered from green fluorescent proteins.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  3. The R882H substitution in the humanThe Journal of biological chemistry · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Curran OiDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520.ORCID 0000-0003-0911-0292
John D TreadoIntegrated Graduate Program in Physical and Engineering Biology, Yale University, New Haven, Connecticut, 06520.
Zachary A LevineDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520.
Christopher S LimDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520.
Kirsten M KnechtDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520.
Yong XiongDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520.
Corey S O'HernIntegrated Graduate Program in Physical and Engineering Biology, Yale University, New Haven, Connecticut, 06520.
Lynne ReganDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520.

Funding

Overall EvaluationP41GM103403 · NIGMS · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2012 to 2017
$14.3M
PREDOCTORAL PROGRAM IN BIOPHYSICST32GM008283 · NIGMS · YALE UNIVERSITY · PI XIONG, YONG · 1988 to 2022
$10.5M
Systems analysis of phenotypic switch in control of cancer invasionU54CA209992 · NCI · YALE UNIVERSITY · PI LEVCHENKO, ANDRE · 2016 to 2021
$10.1M
Recognition of Viral DNA by APOBEC3 Proteins and their Antagonization by HIV VifR01AI116313 · NIAID · YALE UNIVERSITY · PI XIONG, YONG · 2015 to 2019
$2.1M
Pixel Array Detector for Macromolecular CrystallographyS10OD021527 · OD · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2016 to 2016
$2.0M
Pixel Array Detector for X-ray CrystallographyS10RR029205 · NCRR · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2010 to 2010
$1.8M
Designed proteins to study and modulate cellular processesR01GM118528 · NIGMS · YALE UNIVERSITY · PI MOCHRIE, SIMON · 2017 to 2020
$1.2M
Convergent Graduate Training in Engineering, Physics and BiologyT32EB019941 · NIBIB · YALE UNIVERSITY · PI O'HERN, COREY · 2016 to 2020
$998k
NCI NIH HHS U54 CA209992NCRR NIH HHS S10 RR029205NIBIB NIH HHS T32 EB019941NIGMS NIH HHS P41 GM103403NIGMS NIH HHS R01 GM118528NIGMS NIH HHS T32 GM008283NIH HHS S10 OD021527NIH HHS T32GM008283
6 · The paper itself

Abstract

We present the structure of an engineered protein-protein interface between two beta barrel proteins, which is mediated by interactions between threonine (Thr) residues. This Thr zipper structure suggests that the protein interface is stabilized by close-packing of the Thr residues, with only one intermonomer hydrogen bond (H-bond) between two of the Thr residues. This Thr-rich interface provides a unique opportunity to study the behavior of Thr in the context of many other Thr residues. In previous work, we have shown that the side chain (χ

Indexed as

CrystallizationEscherichia coliHydrogen BondingHydrophobic and Hydrophilic InteractionsMolecular Dynamics SimulationProtein BindingProtein ConformationProtein EngineeringProteinsSolventsSurface PropertiesThreonineProteinsSolventsThreoninehard sphere plus stereochemical constraint modelmolecular dynamics simulationspackingprotein-protein interactionprotein structuresteric interactionsthreonine

Identifiers

PMID30198622
PMCPMC6201716

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.