ArticleJournal of molecular biology2018
Molecular Basis for Immunity Protein Recognition of a Type VII Secretion System Exported Antibacterial Toxin.
Article in Journal of molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- An endonuclease V protectsbioRxiv : the preprint server for biology · 2026Article
- A toxic effector of T7SS facilitates bacterial competition and virulence through membrane damage in Streptococcus suis.Veterinary research · 2026Article
- Systematic analysis of the type VII secretion system inFrontiers in microbiology · 2026Article
- Distinct immunity protein families mediate compartment-specific neutralization of a bacterial toxin.Cell reports · 2025Article
- A LXG toxin stabilized by DUF4176 contributes to Streptococcus suis competition and pathogenicity.BMC biology · 2025Article
- A widespread family of molecular chaperones promotes the intracellular stability of type VIIb secretion system-exported toxins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Auto-aggregation inmBio · 2025Article
- Structure of a tripartite protein complex that targets toxins to the type VII secretion system.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The role of proteinaceous toxins secreted byFEMS microbes · 2024Review
- Article
- A type VII-secreted lipase toxin with reverse domain arrangement.Nature communications · 2023Article
- Interbacterial competition mediated by the type VIIb secretion system.Microbiology (Reading, England) · 2023Review
- Characterization of TelE, a T7SS LXG Effector Exhibiting a Conserved C-Terminal Glycine Zipper Motif Required for Toxicity.Microbiology spectrum · 2023Article
- Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract.Molecular microbiology · 2023Article
- A pathogenicity locus of Streptococcus gallolyticus subspecies gallolyticus.Scientific reports · 2023Article
- Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract.bioRxiv : the preprint server for biology · 2023Article
- Homologous recombination between tandem paralogues drives evolution of a subset of type VII secretion system immunity genes in firmicute bacteria.Microbial genomics · 2022Article
- Products of gut microbial Toll/interleukin-1 receptor domain NADase activities in gnotobiotic mice and Bangladeshi children with malnutrition.Cell reports · 2022Article
- Genomic discovery and structural dissection of a novel type of polymorphic toxin system in gram-positive bacteria.Computational and structural biotechnology journal · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Gram-positive bacteria deploy the type VII secretion system (T7SS) to facilitate interactions between eukaryotic and prokaryotic cells. In recent work, we identified the TelC protein from Streptococcus intermedius as a T7SS-exported lipid II phosphatase that mediates interbacterial competition. TelC exerts toxicity in the inner wall zone of Gram-positive bacteria; however, intercellular intoxication of sister cells does not occur because they express the TipC immunity protein. In the present study, we sought to characterize the molecular basis of self-protection by TipC. Using sub-cellular localization and protease protection assays, we show that TipC is a membrane protein with an N-terminal transmembrane segment and a C-terminal TelC-inhibitory domain that protrudes into the inner wall zone. The 1.9-Å X-ray crystal structure of a non-protective TipC paralogue reveals that the soluble domain of TipC proteins adopts a crescent-shaped fold that is composed of three α-helices and a seven-stranded β-sheet. Subsequent homology-guided mutagenesis demonstrates that a concave surface formed by the predicted β-sheet of TipC is required for both its interaction with TelC and its TelC-inhibitory activity. S. intermedius cells lacking the tipC gene are susceptible to growth inhibition by TelC delivered between cells; however, we find that the growth of this strain is unaffected by endogenous or overexpressed TelC, although the toxin accumulates in culture supernatants. Together, these data indicate that the TelC-inhibitory activity of TipC is only required for intercellularly transferred TelC and that the T7SS apparatus transports TelC across the cell envelope in a single step, bypassing the cellular compartment in which it exerts toxicity en route.
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Registered trials
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