Evidence map›Paper›PMID 30194891›Full record

ArticleChembiochem : a European journal of chemical biology2018

An RNase P-Based Assay for Accurate Determination of the 5'-Deoxy-5'-azidoguanosine-Modified Fraction of in Vitro-Transcribed RNAs.

Seth E Lyon, Tien-Hao Chen, Andrew J Wallace, Katie Adib, Venkat Gopalan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Seth E LyonDepartment of Chemistry and Biochemistry and, Center for RNA Biology, The Ohio State University, 774 Biological Sciences Building, 484 West 12th Avenue, Columbus, OH, 43210, USA.
Tien-Hao ChenDepartment of Chemistry and Biochemistry and, Center for RNA Biology, The Ohio State University, 774 Biological Sciences Building, 484 West 12th Avenue, Columbus, OH, 43210, USA.
Andrew J WallaceDepartment of Chemistry and Biochemistry and, Center for RNA Biology, The Ohio State University, 774 Biological Sciences Building, 484 West 12th Avenue, Columbus, OH, 43210, USA.
Katie AdibDepartment of Chemistry and Biochemistry and, Center for RNA Biology, The Ohio State University, 774 Biological Sciences Building, 484 West 12th Avenue, Columbus, OH, 43210, USA.
Venkat GopalanDepartment of Chemistry and Biochemistry and, Center for RNA Biology, The Ohio State University, 774 Biological Sciences Building, 484 West 12th Avenue, Columbus, OH, 43210, USA.ORCID 0000-0002-3016-2719
The Ohio State University · USUniversity of California, Santa Cruz · USWright State University · USYale University · US

Funding

Skill Development for Diverse Scientific Careers: A New Course Predoctoral Program in Cellular and Molecular BiologyT32GM007223 · NIGMS · YALE UNIVERSITY · PI BASERGA, SUSAN J · 1985 to 2019
$31.0M
Dissecting functional cooperation among subunits in a catalytic ribonucleoproteinR01GM120582 · NIGMS · OHIO STATE UNIVERSITY · PI GOPALAN, VENKAT · 2016 to 2019
$1.9M
Typhoon FLA9500 Biomedical ImagerS10OD023582 · OD · OHIO STATE UNIVERSITY · PI JACKMAN, JANE ELIZABETH · 2017 to 2017
$144k
NIGMS NIH HHS R01 GM120582NIGMS NIH HHS T32 GM007223NIH HHS S10 OD023582
6 · The paper itself

Abstract

Chemoenzymatic approaches are important for generating site-specific, chemically modified RNAs, a cornerstone for RNA structure-function correlation studies. T7 RNA polymerase (T7RNAP)-mediated in vitro transcription (IVT) of a DNA template containing the G-initiating class III Φ6.5 promoter is typically used to generate 5'-chemically modified RNAs by including a guanosine analogue (G analogue) initiator in the IVT. However, the yield of 5'-G analogue-initiated RNA is often poor and variable due to the high ratios of G analogue:GTP used in IVT. We recently reported that a T7RNAP P266L mutant afforded an approximately three-fold increase in fluorescent 5'-thienoguanosine-initiated pre-tRNA

Indexed as

Transcription, GeneticDNA-Directed RNA PolymerasesGuanosineMutationPromoter Regions, GeneticRibonuclease PRNA PrecursorsViral Proteinsbacteriophage T7 RNA polymeraseDNA-Directed RNA PolymerasesGuanosineRibonuclease PRNA PrecursorsViral Proteins5′ modificationazidoguanosinein vitro transcriptionRNA

Identifiers

PMID30194891
PMCPMC6392026
OpenAlexW2892079079

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.