Evidence map›Paper›PMID 30194483›Full record

ArticleMicrobial ecology2019

High-Throughput Sequencing Analysis of the Bacterial Community in Stone Fruit Phloem Tissues Infected by "Candidatus Phytoplasma prunorum".

Ales Eichmeier, Tomas Kiss, Tomas Necas, Eliska Penazova, Dorota Tekielska, Marketa Bohunicka, Lucie Valentova, Radek Cmejla, Daniel Morais, Petr Baldrian

Abstract read
PubMed Publisher
In one paragraph

Article in Microbial ecology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Molecular Characterization of 'Microorganisms · 2024
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Ales EichmeierMendeleum - Institute of Genetics, Mendel University in Brno, Valtická 334, 691 44, Lednice, Czech Republic. ales.eichmeier@mendelu.cz.
Tomas KissDepartment of Fruit Growing, Mendel University in Brno, Valtická 337, 691 44, Lednice, Czech Republic.
Tomas NecasDepartment of Fruit Growing, Mendel University in Brno, Valtická 337, 691 44, Lednice, Czech Republic.
Eliska PenazovaMendeleum - Institute of Genetics, Mendel University in Brno, Valtická 334, 691 44, Lednice, Czech Republic.
Dorota TekielskaDepartment of Plant Protection, University of Agriculture in Krakow, 29 Listopada 54, 31-425, Krakow, Poland.
Marketa BohunickaDepartment of Biology, Faculty of Science, University of Hradec Králové, Rokitanského 62, 500 03, Hradec Králové, Czech Republic.
Lucie ValentovaResearch and Breeding Institute of Pomology Holovousy Ltd, Holovousy 129, 508 01, Hořice, Czech Republic.
Radek CmejlaResearch and Breeding Institute of Pomology Holovousy Ltd, Holovousy 129, 508 01, Hořice, Czech Republic.
Daniel MoraisLaboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220, Praha 4, Czech Republic.
Petr BaldrianLaboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220, Praha 4, Czech Republic.
Mendel University in Brno · CZCzech Academy of Sciences, Institute of Microbiology · CZResearch and Breeding Institute of Pomology Holovousy · CZUniversity of Agriculture in Krakow · PLUniversity of Hradec Králové · CZ

Funding

Ministerstvo Školství, Mládeže a Tělovýchovy CZ.02.1.01/0.0/0.0/16_025/0007314Ministerstvo Školství, Mládeže a Tělovýchovy LM2015042Ministry of Agriculture of the Czech Republic QJ1510352
6 · The paper itself

Abstract

"Candidatus Phytoplasma prunorum" (CPp) is a highly destructive phytopathogenic agent in many stone fruit-growing regions in Europe and the surrounding countries. In this work, we focused on documenting entire bacterial community in the phloem tissues of 60 stone fruit trees. Nested PCR and two real-time PCR assays were used to select CPp-positive (group A) and CPp-negative samples (group B). Afterwards, high-throughput amplicon sequencing was performed to assess bacterial community compositions in phloem tissues. The bacterial composition in phloem tissue consisted of 118 distinct genera, represented mainly by Pseudomonas, Acinetobacter, Methylobacterium, Sphingomonas, and Rhizobium. Statistics showed that CPp influenced the bacterial composition of infected plants (group A) and that the bacterial community depended on the geographical origin of the sample. This is the first work focusing on an analysis of the influence of CPp on the bacteria coexisting in the phloem tissues of stone fruit trees.

Indexed as

BacteriaBiodiversityFruitHigh-Throughput Nucleotide SequencingPhloemPhytoplasmaPlant DiseasesPrunusBacterial composition“Candidatus Phytoplasma prunorum”High-throughput sequencingPhloemStone Fruit

Identifiers

PMID30194483
OpenAlexW2890150059

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.