ArticleNature communications2018
Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Multimodal techniques based on synchrotron radiation X-rays for revealing interactions of metallic nanoparticles with biological matrices.Fundamental research · 2026Review
- Sample Preparation Protocol for Laboratory Cryo-Soft X-Ray Microscopy for Studying Cellular Nanoparticle Uptake.International journal of molecular sciences · 2025Article
- Three-dimensional structure of entire hydrated murine hearts at histological resolution.Scientific reports · 2025Article
- Sarcomere, troponin, and myosin X-ray diffraction signals can be resolved in single cardiomyocytes.Biophysical journal · 2024Article
- In situ label-free X-ray imaging for visualizing the localization of nanomedicines and subcellular architecture in intact single cells.Nature protocols · 2024Review
- Correlative Raman-Electron-Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells.Biosensors · 2022Article
- Combined optical fluorescence microscopy and X-ray tomography reveals substructures in cell nuclei in 3D.Biomedical optics express · 2022Article
- Advanced Light Source Analytical Techniques for Exploring the Biological Behavior and Fate of Nanomedicines.ACS central science · 2022Review
- Scanning x-ray microdiffraction: In situ molecular imaging of tissue and materials.Current opinion in structural biology · 2022Review
- Revealing Inflammatory Indications Induced by Titanium Alloy Wear Debris in Periprosthetic Tissue by Label-Free Correlative High-Resolution Ion, Electron and Optical Microspectroscopy.Materials (Basel, Switzerland) · 2021Article
- Combined scanning small-angle X-ray scattering and holography probes multiple length scales in cell nuclei.Journal of synchrotron radiation · 2021Article
- Article
- X-ray diffraction and second harmonic imaging reveal new insights into structural alterations caused by pressure-overload in murine hearts.Scientific reports · 2020Article
- X-Ray Structural Analysis of Single Adult Cardiomyocytes: Tomographic Imaging and Microdiffraction.Biophysical journal · 2020Article
- Multi-scale X-ray phase-contrast tomography of murine heart tissue.Biomedical optics express · 2020Article
- Macro-to-nanoscale investigation of wall-plate joints in the acorn barnacle Semibalanus balanoides: correlative imaging, biological form and function, and bioinspiration.Journal of the Royal Society, Interface · 2019Article
- Holography applications toward medical field: An overview.The Indian journal of radiology & imagingArticle
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
We present a correlative microscopy approach for biology based on holographic X-ray imaging, X-ray scanning diffraction, and stimulated emission depletion (STED) microscopy. All modalities are combined into the same synchrotron endstation. In this way, labeled and unlabeled structures in cells are visualized in a complementary manner. We map out the fluorescently labeled actin cytoskeleton in heart tissue cells and superimpose the data with phase maps from X-ray holography. Furthermore, an array of local far-field diffraction patterns is recorded in the regime of small-angle X-ray scattering (scanning SAXS), which can be interpreted in terms of biomolecular shape and spatial correlations of all contributing scattering constituents. We find that principal directions of anisotropic diffraction patterns coincide to a certain degree with the actin fiber directions and that actin stands out in the phase maps from holographic recordings. In situ STED recordings are proposed to formulate models for diffraction data based on co-localization constraints.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.