Evidence map›Paper›PMID 30194355›Full record

ArticleScientific reports2018

Transcriptome analysis of differentially expressed unigenes involved in flavonoid biosynthesis during flower development of Chrysanthemum morifolium 'Chuju'.

Junyang Yue, Chuanxue Zhu, Yu Zhou, Xiangli Niu, Min Miao, Xiaofeng Tang, Fadi Chen, Weiping Zhao, Yongsheng Liu

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
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  7. Article
  8. Genome-Wide Analysis ofPlants (Basel, Switzerland) · 2024
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  14. Article
  15. Characterization ofFrontiers in plant science · 2022
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Junyang YueSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Chuanxue ZhuSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Yu ZhouSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Xiangli NiuSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Min MiaoSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Xiaofeng TangSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Fadi ChenCollege of Horticulture, Key Laboratory of Landscape Agriculture, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
Weiping ZhaoSchool of Biological Science and Food Engineering, Chuzhou University, Chuzhou, 239000, China. zwp_1020@163.com.
Yongsheng LiuSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China. liuyongsheng1122@hfut.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chrysanthemum morifolium is an ornamentally and medicinally important plant species. Up to date, molecular and genetic investigations have largely focused on determination of flowering time in the ornamental species. However, little is known about gene regulatory networks for the biosynthesis of flavonoids in the medicinal species. In the current study, we employed the high-throughput sequencing technology to profile the genome-wide transcriptome of C. morifolium 'Chuju', a famous medicinal species in traditional Chinese medicine. A total of 63,854 unigenes with an average length of 741 bp were obtained. Bioinformatic analysis has identified a great number of structural and regulatory unigenes potentially participating in the flavonoid biosynthetic pathway. According to the comparison of digital gene expression, 8,370 (3,026 up-regulated and 5,344 down-regulated), 1,348 (717 up-regulated and 631 down-regulated) and 944 (206 up-regulated and 738 down-regulated) differentially expressed unigenes (DEUs) were detected in the early, middle and mature growth phases, respectively. Among them, many DEUs were implicated in controlling the biosynthesis and composition of flavonoids from the budding to full blooming stages during flower development. Furthermore, the expression patterns of 12 unigenes involved in flavonoid biosynthesis were generally validated by using quantitative real time PCR. These findings could shed light on the molecular basis of flavonoid biosynthesis in C. morifolium 'Chuju' and provide a genetic resource for breeding varieties with improved nutritional quality.

Indexed as

TranscriptomeChrysanthemumFlavonoidsFlowersGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantGenes, PlantFlavonoids

Identifiers

PMID30194355
PMCPMC6128863

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.