ArticleScientific reports2018
Establishment of keratinocyte cell lines from human hair follicles.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Kit-based remote collection and isolation of human reprogrammable somatic cells for generation of induced pluripotent stem cells and cerebral organoids.Nature protocols · 2026Review
- Keratinocytes with DNA Aberration Induced by UVB Become Susceptible to Ferroptosis.Antioxidants (Basel, Switzerland) · 2026Article
- Proteome of Human Scalp Hair Follicles.Cureus · 2026Article
- SIRT6 enhances the therapeutic potential of extracellular vesicles in mitigating osteoarthritis in rat models.Stem cell research & therapy · 2026Article
- Combining advanced 3D spheroid-based skin models with deep-learning-based image analysis enables in-depth investigation of keratinocyte differentiation and barrier function.Frontiers in bioengineering and biotechnology · 2026Article
- Establishment of immortalized human minor salivary gland cells and chemokine expression induced by IFN-γ, TNF-α, and IL-1β.Odontology · 2025Article
- Characterizing SV40-hTERT Immortalized Human Lung Microvascular Endothelial Cells as Model System for Mechanical Stretch-Induced Lung Injury.International journal of molecular sciences · 2025Article
- Type 2 Cytokine-Dependent Skin Barrier Regulation in Personalized 2-Dimensional and 3-Dimensional Skin Models of Atopic Dermatitis: A Pilot Study.JID innovations : skin science from molecules to population health · 2025Article
- Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation.Biomolecules · 2024Article
- Cornification of keratinocytes is associated with differential changes in the catalytic activity and the immunoreactivity of transglutaminase-1.Scientific reports · 2023Article
- Review
- The proliferative and multipotent epidermal progenitor cells for human skin reconstruction in vitro and in vivo.Cell proliferation · 2022Article
- DNA hypomethylation leads to cGAS-induced autoinflammation in the epidermis.The EMBO journal · 2021Article
- miR-155 Contributes to Normal Keratinocyte Differentiation and Is Upregulated in the Epidermis of Psoriatic Skin Lesions.International journal of molecular sciences · 2020Article
- Analysis of histological and microRNA profiles changes in rabbit skin development.Scientific reports · 2020Article
- Review
- CD46 knock-out using CRISPR/Cas9 editing of hTERT immortalized human cells modulates complement activation.PloS one · 2019Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The advent of organotypic skin models advanced the understanding of complex mechanisms of keratinocyte differentiation. However, these models are limited by both availability of primary keratinocytes and donor variability. Keratinocytes derived from cultured hair follicles and interfollicular epidermis were immortalized by ectopic expression of SV40 and hTERT. The generated keratinocyte cell lines differentiated into stratified epidermis with well-defined stratum granulosum and stratum corneum in organotypic human skin models. They behaved comparable to primary keratinocytes regarding the expression of differentiation-associated proteins, cell junction components and proteins associated with cornification and formed a barrier against biotin diffusion. Mechanistically, we found that SV40 large T-antigen expression, accompanied by a strong p53 accumulation, was only detectable in the basal layer of the in vitro reconstructed epidermis. Inhibition of DNA-methylation resulted in expression of SV40 large T-antigen also in the suprabasal epidermal layers and led to incomplete differentiation of keratinocyte cell lines. Our study demonstrates the generation of keratinocyte cell lines which are able to fully differentiate in an organotypic skin model. Since hair follicles, as source for keratinocytes, can be obtained by minimally invasive procedures, our approach enables the generation of cell lines also from individuals not available for skin biopsies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.