ArticleScientific reports2018
X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
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- Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging.Nature methods · 2023Article
- A reliable workflow for improving nanoscale X-ray fluorescence tomographic analysis on nanoparticle-treated HeLa cells.Metallomics : integrated biometal science · 2022Article
- Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline.Journal of synchrotron radiation · 2022Article
- Synchrotron-Based Nano-X-Ray Absorption Near-Edge Structure Revealing Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria.Small science · 2022Article
- Dual energy X-ray beam ptycho-fluorescence imaging.Journal of synchrotron radiation · 2021Article
- Development of Multi-Scale X-ray Fluorescence Tomography for Examination of Nanocomposite-Treated Biological Samples.Cancers · 2021Article
- Off-axis multilayer zone plate with 16 nm × 28 nm focus for high-resolution X-ray beam induced current imaging.Journal of synchrotron radiation · 2021Article
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- Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.Nature communications · 2020Article
- Advances in visualization of copper in mammalian systems using X-ray fluorescence microscopy.Current opinion in chemical biology · 2020Review
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- Spectroscopic imaging with single acquisition ptychography and a hyperspectral detector.Scientific reports · 2019Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
X-ray Fluorescence (XRF) microscopy is a growing approach for imaging the trace element concentration, distribution, and speciation in biological cells at the nanoscale. Moreover, three-dimensional nanotomography provides the added advantage of imaging subcellular structure and chemical identity in three dimensions without the need for staining or sectioning of cells. To date, technical challenges in X-ray optics, sample preparation, and detection sensitivity have limited the use of XRF nanotomography in this area. Here, XRF nanotomography was used to image the elemental distribution in individual E. coli bacterial cells using a sub-15 nm beam at the Hard X-ray Nanoprobe beamline (HXN, 3-ID) at NSLS-II. These measurements were simultaneously combined with ptychography to image structural components of the cells. The cells were embedded in small (3-20 µm) sodium chloride crystals, which provided a non-aqueous matrix to retain the three-dimensional structure of the E. coli while collecting data at room temperature. Results showed a generally uniform distribution of calcium in the cells, but an inhomogeneous zinc distribution, most notably with concentrated regions of zinc at the polar ends of the cells. This work demonstrates that simultaneous two-dimensional ptychography and XRF nanotomography can be performed with a sub-15 nm beam size on unfrozen biological cells to co-localize elemental distribution and nanostructure simultaneously.
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