ArticleG3 (Bethesda, Md.)2018
Bulked-Segregant Analysis Coupled to Whole Genome Sequencing (BSA-Seq) for Rapid Gene Cloning in Maize.
Article in G3 (Bethesda, Md.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- FaNAC29: a novel gene positively regulating strawberry powdery mildew resistance.The New phytologist · 2026Article
- BloomSight: An ultra-high-frequency phenotyping framework for diurnal flowering dynamics inPlant phenomics (Washington, D.C.) · 2026Article
- Quick creation and mapping of EMS-induced maize kernel mutants identifies classical gene ZmBT1 and novel gene ZmTOP6A.The plant genome · 2026Article
- Harnessing Bulk-Segregant Mapping to Identify Trait-Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- The Genetic Loci Associated with Fiber Development in Upland Cotton (Plants (Basel, Switzerland) · 2025Article
- Targeted seed EMS mutagenesis reveals a basic helix-loop-helix transcription factor underlying male sterility in sorghum.Genetics · 2025Article
- Phenotype to genotype: A new and rapid approach using whole-genome sequencing.PLoS genetics · 2025Article
- The Identification of a Single-Base Mutation in the MaizePlants (Basel, Switzerland) · 2025Article
- Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants.Nature plants · 2025Article
- Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress inInternational journal of molecular sciences · 2025Article
- Novel Allelic Mutations inInternational journal of molecular sciences · 2024Article
- Identification of Dwarfing Candidate Genes inPlants (Basel, Switzerland) · 2024Article
- Function Analysis of a Maize Endo-1,4-β-xylanase GeneInternational journal of molecular sciences · 2024Article
- Identification of candidate genes controlling cold tolerance at the early seedling stage from Dongxiang wild rice by QTL mapping, BSA-Seq and RNA-Seq.BMC plant biology · 2024Article
- The H3K4 demethylase JMJ1 is required for proper timing of flowering in Brachypodium distachyon.The Plant cell · 2024Article
- Genetic characterization of a locus responsible for low pungency using EMS-induced mutants in Capsicum annuum L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Article
- Exploration into Natural Variation Genes Associated with Determinate and Capitulum-like Inflorescence inInternational journal of molecular sciences · 2023Article
- Identification of salt stress-tolerant candidate genes in the BCFrontiers in plant science · 2023Article
- Versatile mapping-by-sequencing with Easymap v.2.Frontiers in plant science · 2023Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Forward genetics remains a powerful method for revealing the genes underpinning organismal form and function, and for revealing how these genes are tied together in gene networks. In maize, forward genetics has been tremendously successful, but the size and complexity of the maize genome made identifying mutant genes an often arduous process with traditional methods. The next generation sequencing revolution has allowed for the gene cloning process to be significantly accelerated in many organisms, even when genomes are large and complex. Here, we describe a bulked-segregant analysis sequencing (BSA-Seq) protocol for cloning mutant genes in maize. Our simple strategy can be used to quickly identify a mapping interval and candidate single nucleotide polymorphisms (SNPs) from whole genome sequencing of pooled F2 individuals. We employed this strategy to identify
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