ArticleThe Biochemical journal1986
The membrane topography of ecto-5'-nucleotidase in rat hepatocytes.
Article in The Biochemical journal, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Demonstration of 5'-nucleotidase activity in unfixed cryostat sections of rat liver using a combined light- and electron-microscope procedure.The Histochemical journal · 1995Article
- Adenosine deaminase, 5'-nucleotidase, xanthine oxidase, superoxide dismutase, and catalase activities in gastric juices from patients with gastric cancer, ulcer, and atrophic gastritis.Digestive diseases and sciences · 1994Article
- Isolation and characterization of the ecto-5'-nucleotidase from a rat glioblastoma cell line.Molecular and cellular biochemistry · 1992Article
- Modulation of 5'-nucleotidase activity in plasma membranes and intact cells by the extracellular matrix proteins laminin and fibronectin.The Biochemical journal · 1992Article
- Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles.The Biochemical journal · 1991Article
- Inositol is a constituent of detergent-solubilized immunoaffinity-purified rat liver 5'-nucleotidase.The Biochemical journal · 1990Article
- 5-nucleotidase activity in cultured cell lines. Effect of different assay conditions and correlation with cell proliferation.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1989Article
- 5'-Nucleotidases of chicken gizzard and human pancreatic adenocarcinoma cells are anchored to the plasma membrane via a phosphatidylinositol-glycan.The Biochemical journal · 1989Article
- The pigeon heart 5'-nucleotidase responsible for ischaemia-induced adenosine formation.The Biochemical journal · 1988Article
- A quantitative histochemical study of 5'-nucleotidase activity in rat liver using the lead salt method and polyvinyl alcohol.The Histochemical journal · 1988Article
- A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine.The Biochemical journal · 1988Article
- Purification, characterization and cellular localization of 5'-nucleotidase from Torpedo electric organ.The Biochemical journal · 1987Article
- Enzymes involved in adenosine metabolism in rat white and brown adipocytes. Effects of streptozotocin-diabetes, hypothyroidism, age and sex differences.The Biochemical journal · 1987Article
- The preparation of monoclonal antibodies to human bone and liver alkaline phosphatase and their use in immunoaffinity purification and in studying these enzymes when present in serum.The Biochemical journal · 1987Article
- Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors.The Biochemical journal · 1987Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transmembrane topography of the rat hepatocyte ectoenzyme 5'-nucleotidase was studied by the use of glycoprotein labelling and limited-proteolysis techniques. Comparison, by one-dimensional peptide mapping, of enzyme iodinated from outside the cell with that iodinated in the solubilized state showed that no additional iodination sites were revealed on solubilization. Incubation of newly synthesized enzyme in a microsomal membrane fraction with proteinase showed that the entire molecule of 5'-nucleotidase was protected from proteolysis. These data suggest that little, if any, of the 5'-nucleotidase molecule is present on the cytoplasmic side of the plasma membrane. No evidence was found for a previously proposed interaction between 5'-nucleotidase and actin, although the ability of preparations of 5'-nucleotidase to prevent inhibition of deoxyribonuclease I by actin was explained by minute traces of ATPase activity. Comparison of peptide maps of enzyme labelled by iodination or by methods specific for carbohydrate showed that in both cases predominantly one section of the molecule was labelled. It is proposed that the enzyme is a short-stalked integral membrane protein without a cytoplasmic domain in which about one-third of the molecule forms the accessible molecular surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.