ArticlePloS one2018
Over 2.5 million COI sequences in GenBank and growing.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Article
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- Evaluating the Molecular Potential and Interpretability of DNA in Historical Spirit Collection Media.Molecular ecology resources · 2026Article
- Complete Mitochondrial Genome ofEcology and evolution · 2026Article
- Article
- Phylogeography of the widely distributed John Dory (Zeus faber, Actinopterygii: Zeiformes) reaffirms the prevalence of at least two deeply divergent clades.Journal of fish biology · 2026Article
- DeepCOI: a large language model-driven framework for fast and accurate taxonomic assignment in animal metabarcoding.Genome biology · 2025Article
- Review
- From Mud to Meat: Comparative Metabarcoding Reveals Two Different Evolutionary Paths to Carnivory in a Group of Meiofaunal Annelids.Molecular ecology · 2025Article
- Relative performance of three phylogenetic methods based on complete mitochondrial genomes of barnacle.Scientific reports · 2025Article
- Structure and Composition of Prey Communities Associated with Malaysian Mahseer,Tropical life sciences research · 2025Article
- Diversity and Seasonal Abundance ofInsects · 2025Article
- Article
- Exploring the potential of the COI gene marker for DNA barcoding of planktonic foraminifera.Scientific reports · 2025Article
- Macrogenetics Approach Reveals Spatial Trends and Drivers of Mitochondrial Genetic Diversity at Different Biological Organization Levels in Tropical Western Atlantic Decapods.Ecology and evolution · 2025Article
- A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018-2020.Molecular ecology resources · 2025Article
- Article
- A new genus and species of feather duster worm (Annelida, Sabellidae) from shallow hydrocarbon seeps in the Gulf of Mexico.Biodiversity data journal · 2025Article
- Diversity of lanternfish (Myctophidae) larvae along the Ninety East Ridge, Indian Ocean.PeerJ · 2025Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing popularity of cytochrome c oxidase subunit 1 (COI) DNA metabarcoding warrants a careful look at the underlying reference databases used to make high-throughput taxonomic assignments. The objectives of this study are to document trends and assess the future usability of COI records for metabarcode identification. The number of COI records deposited to the NCBI nucleotide database has increased by a geometric average of 51% per year, from 8,137 records deposited in 2003 to a cumulative total of ~ 2.5 million by the end of 2017. About half of these records are fully identified to the species rank, 92% are at least 500 bp in length, 74% have a country annotation, and 51% have latitude-longitude annotations. To ensure the future usability of COI records in GenBank we suggest: 1) Improving the geographic representation of COI records, 2) Improving the cross-referencing of COI records in the Barcode of Life Data System and GenBank to facilitate consolidation and incorporation into existing bioinformatic pipelines, 3) Adherence to the minimum information about a marker gene sequence guidelines, and 4) Integrating metabarcodes from eDNA and mixed community studies with existing reference sequences. The growth of COI reference records over the past 15 years has been substantial and is likely to be a resource across many fields for years to come.
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