ReviewJournal of industrial microbiology & biotechnology2018
Bacteriophage-resistant industrial fermentation strains: from the cradle to CRISPR/Cas9.
Review in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Firewalled synthetic commensal blocks horizontal gene transfer in the gut.bioRxiv : the preprint server for biology · 2026Article
- Development of phage-resistantFood chemistry: X · 2026Article
- Bacterial defense systems: Mechanisms, homology to eukaryotic immune systems, and applications.Zoological research · 2026Review
- A CRISPR-Cas9 system protecting E. coli against acquisition of antibiotic resistance genes.Scientific reports · 2025Article
- Evaluation of Cellular Responses of HeterotrophicMicroorganisms · 2024Article
- Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook.Pathogens (Basel, Switzerland) · 2024Review
- Tn5 Transposon-based Mutagenesis for Engineering Phage-resistant Strains of Escherichia coli BL21 (DE3).Journal of microbiology (Seoul, Korea) · 2023Article
- Anionic Synthetic Polymers Prevent Bacteriophage Infection.Journal of the American Chemical Society · 2023Article
- Article
- Characterization, complete genome sequencing, and CRISPR/Cas9 system-based decontamination of a novelFrontiers in microbiology · 2023Article
- Peptide-Grafted Nontoxic Cyclodextrins and Nanoparticles against Bacteriophage Infections.ACS nano · 2022Article
- High-Titer Production of the Fungal Anhydrotetracycline, TAN-1612, in Engineered Yeasts.ACS synthetic biology · 2022Article
- Theragnostic application of nanoparticle and CRISPR against food-borne multi-drug resistant pathogens.Materials today. Bio · 2022Review
- Engineering laboratory/factory-specific phage-resistant strains of Escherichia coli by mutagenesis and screening.World journal of microbiology & biotechnology · 2022Article
- An Alternative to Antibiotics: Selected Methods to Combat Zoonotic Foodborne Bacterial Infections.Current microbiology · 2021Review
- Broad-spectrum nanoparticles against bacteriophage infections.Nanoscale · 2021Article
- Recent advances in n-butanol and butyrate production using engineered Clostridium tyrobutyricum.World journal of microbiology & biotechnology · 2020Review
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophage contamination and cell lysis have been recurring issues with some actinomycetes used in the pharmaceutical fermentation industry since the commercialization of streptomycin in the 1940s. In the early years, spontaneous phage-resistant mutants or lysogens were isolated to address the problem. In some cases, multiple phages were isolated from different contaminated fermentors, so strains resistant to multiple phages were isolated to stabilize the fermentation processes. With the advent of recombinant DNA technology, the early scaleup of the Escherichia coli fermentation process for the production of human insulin A and B chains encountered contamination with multiple coliphages. A genetic engineering solution was to clone and express a potent restriction/modification system in the production strains. Very recently, an E. coli fermentation of 1,3-propanediol was contaminated by a coliphage related to T1. CRISPR/Cas9 technology was applied to block future contamination by targeting seven different phage genes for double-strand cleavage. These approaches employing spontaneous mutation, genetic engineering, and synthetic biology can be applied to many current and future microorganisms used in the biotechnology industry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.