Evidence map›Paper›PMID 30191309›Full record

ArticleParasitology research2018

Identification of a perchloric acid-soluble protein (PSP)-like ribonuclease from Trichomonas vaginalis.

Alma Villalobos-Osnaya, Georgina Garza-Ramos, Iris N Serratos, César Millán-Pacheco, Arturo González-Robles, Rossana Arroyo, Laura Itzel Quintas-Granados, María Elizbeth Alvarez-Sanchez

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In one paragraph

Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 1 country.

Alma Villalobos-OsnayaPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, 03100, Ciudad de Mexico, Mexico.
Georgina Garza-RamosDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Universidad # 3000, 04510, Ciudad de Mexico, Mexico.
Iris N SerratosDepartamento de Química, Universidad Autónoma Metropolitana, Apartado Postal 55-534, Iztapalapa, 09340, Ciudad de Mexico, Mexico.
César Millán-PachecoFacultad de Farmacia, Universidad Autónoma del Estado de Morelos, 62209, Cuernavaca, Morelos, Mexico.
Arturo González-RoblesDepartamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN # 2508, Col. San Pedro Zacatenco, Delg. Gustavo A. Madero, 07360, Ciudad de Mexico, Mexico.
Rossana ArroyoDepartamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN # 2508, Col. San Pedro Zacatenco, Delg. Gustavo A. Madero, 07360, Ciudad de Mexico, Mexico.
Laura Itzel Quintas-GranadosUniversidad Mexiquense del Bicentenario, Unidad de Estudios Superiores Tultitlan, Av. Ex Hacienda de Portales s/n, Col. Villa Esmeralda, 54910, Tultitlan, Estado de México, Mexico.
María Elizbeth Alvarez-SanchezPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, 03100, Ciudad de Mexico, Mexico. elizbeth2@gmail.com.ORCID http://orcid.org/0000-0002-9212-4451
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional · MXUniversidad Autónoma de la Ciudad de México · MXEl Colegio Mexiquense · MXUniversidad Autónoma del Estado de Morelos · MXUniversidad Autónoma Metropolitana · MXUniversidad Nacional Autónoma de México · MX

Funding

CONACYT 83808
6 · The paper itself

Abstract

A perchloric acid-soluble protein (PSP), named here tv-psp1, was identified in Trichomonas vaginalis. It is expressed under normal culture conditions according to expressed sequence tag (EST) analysis. On the other hand, Tv-PSP1 protein was identified by mass spectrometry with a 40% of identity to human PSP (p14.1). Polyclonal antibodies against recombinant Tv-PSP1 (rTv-PSP1) recognized a single band at 13.5 kDa in total protein parasite extract by SDS-PAGE and a high molecular weight band analyzed by native PAGE. Structural analysis of Tv-PSP1, using dynamic light scattering, size exclusion chromatography, and circular dichroism spectroscopy, showed a trimeric structure stable at 7 M urea with 38% α-helix and 14% β-sheet in solution and a molecular weight of 40.5 kD. Tv-PSP1 models were used to perform dynamic simulations over 100 ns suggesting a stable homotrimeric structure. Tv-PSP1 was located in the nucleus, cytoplasm, and hydrogenosomes of T. vaginalis, and the in silico analysis by Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) showed interactions with RNA binding proteins. The preliminary results of RNA degradation analysis with the recombinant Tv-PSP1 showed RNA partial deterioration suggesting a possible putative ribonuclease function.

Indexed as

Amino Acid SequenceAnimalsCircular DichroismElectrophoresis, Polyacrylamide GelHeat-Shock ProteinsHumansMiceMice, Inbred BALB CMolecular Dynamics SimulationPerchloratesProtozoan ProteinsRibonucleasesRNA-Binding ProteinsTrichomonas vaginalisHeat-Shock ProteinsPerchloratesPerchloric AcidProtozoan ProteinsRibonucleasesRIDA protein, humanRNA-Binding ProteinsCellular localizationHomotrimeric structureMolecular dynamicPerchloric acid-soluble proteinsSize exclusion chromatographicTrichomonas vaginalis

Identifiers

PMID30191309
OpenAlexW2891640609

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.