ArticleScientific reports2018
The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Fifteen Years after the Definition ofLife (Basel, Switzerland) · 2023Review
- Differential expression of meiosis and homologous recombination-related genes in the life cycle of Trypanosoma cruzi.Parasitology research · 2023Article
- Divergent binding mode for a protozoan BRC repeat to RAD51.The Biochemical journal · 2022Article
- Experimental Hybridization inPathogens (Basel, Switzerland) · 2022Review
- Microevolution ofeLife · 2022Article
- Stress conditions promoteeLife · 2022Article
- Article
- Sex in protists: A new perspective on the reproduction mechanisms of trypanosomatids.Genetics and molecular biology · 2022Article
- Molecular and Clinical Aspects of Chronic Manifestations in Chagas Disease: A State-of-the-Art Review.Pathogens (Basel, Switzerland) · 2021Review
- Reproduction in Trypanosomatids: Past and Present.Biology · 2021Review
- DNA Double-Strand Breaks: A Double-Edged Sword for Trypanosomatids.Frontiers in cell and developmental biology · 2021Review
- Conditional knockout of RAD51-related genes in Leishmania major reveals a critical role for homologous recombination during genome replication.PLoS genetics · 2020Article
- Evidence of hybridization, mitochondrial introgression and biparental inheritance of the kDNA minicircles in Trypanosoma cruzi I.PLoS neglected tropical diseases · 2020Article
- The Influence of Recombinational Processes to Induce Dormancy inFrontiers in cellular and infection microbiology · 2020Article
- DNA lesions and repair in trypanosomatids infection.Genetics and molecular biology · 2020Article
- Transcriptomic changes across the life cycle ofPeerJ · 2020Article
- Elucidating diversity in the class composition of the minicircle hypervariable region of Trypanosoma cruzi: New perspectives on typing and kDNA inheritance.PLoS neglected tropical diseases · 2019Article
- Analysis of DNA Exchange Using Thymidine Analogs (ADExTA) inBio-protocol · 2018Article
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of genetic exchange has been a limiting factor to deepen the knowledge on the mechanisms by which Trypanosoma cruzi is able to generate progeny and genetic diversity. Here we show that incorporation of halogenated thymidine analogues, followed by immunostaining, is a reliable method not only to detect T. cruzi fused-cell hybrids, but also to quantify their percentage in populations of this parasite. Through this approach, we were able to detect and quantify fused-cell hybrids of T. cruzi clones CL Brener and Y. Given the increased detection of fused-cell hybrids in naturally-occurring hybrid CL Brener strain, which displays increased levels of RAD51 and BRCA2 transcripts, we further investigated the role of Rad51 - a recombinase involved in homologous recombination - in the process of genetic exchange. We also verified that the detection of fused-cell hybrids in T. cruzi overexpressing RAD51 is increased when compared to wild-type cells, suggesting a key role for Rad51 either in the formation or in the stabilization of fused-cell hybrids in this organism.
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