ArticleScientific reports2018
Targeted enrichment outperforms other enrichment techniques and enables more multi-species RNA-Seq analyses.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- A Meta-Analysis ofG3 (Bethesda, Md.) · 2020Pooled it
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- The HosA histone deacetylase regulates stress resistance, host cell interactions, and virulence inbioRxiv : the preprint server for biology · 2025Article
- Transcript-Capture sequencing enriches mRNA ofbioRxiv : the preprint server for biology · 2025Article
- Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiontMicrobial genomics · 2025Article
- Wb5, a novel biomarker for monitoring efficacy and success of mass drug administration programs for Wuchereria bancrofti elimination.PLoS neglected tropical diseases · 2025Article
- Evaluating methods for genome sequencing ofMicrobial genomics · 2025Article
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- Epidermal Growth Factor Receptor Signaling Governs the Host Inflammatory Response to Invasive Aspergillosis.bioRxiv : the preprint server for biology · 2024Article
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- Common analysis of direct RNA sequencinG CUrrently leads to misidentification of mLife science alliance · 2024Article
- Multiplexed target enrichment of coding and non-coding transcriptomes enables studyingFrontiers in cellular and infection microbiology · 2023Article
- UsingVirulence · 2022Article
- Best practices on the differential expression analysis of multi-species RNA-seq.Genome biology · 2021Review
- Long-read RNA sequencing of human and animal filarial parasites improves gene models and discovers operons.PLoS neglected tropical diseases · 2020Article
- Understanding Mucormycoses in the Age of "omics".Frontiers in genetics · 2020Review
- Article
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 1 country.
Funding
Abstract
Enrichment methodologies enable the analysis of minor members in multi-species transcriptomic data. We compared the standard enrichment of bacterial and eukaryotic mRNA to a targeted enrichment using an Agilent SureSelect (AgSS) capture for Brugia malayi, Aspergillus fumigatus, and the Wolbachia endosymbiont of B. malayi (wBm). Without introducing significant systematic bias, the AgSS quantitatively enriched samples, resulting in more reads mapping to the target organism. The AgSS-enriched libraries consistently had a positive linear correlation with their unenriched counterparts (r
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