ArticleNature communications2018
Utilization of rare codon-rich markers for screening amino acid overproducers.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Engineering of a LysG-derived arginine-specific biosensor for high-throughput screening of arginine overproducers in Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2026Article
- Rare-codon-tuned fluorescent biosensor for high-throughput selection of Escherichia coli strain capable of L-arginine overproduction.BMC microbiology · 2026Article
- Decoupled tricarboxylic acid cycle and glycolysis ofSynthetic and systems biotechnology · 2025Article
- Combining biosensor and metabolic network optimization strategies for enhanced L-threonine production in Escherichia coli.Biotechnology for biofuels and bioproducts · 2025Article
- Screening of high glucose tolerant Escherichia coli for L-valine fermentation by autonomous evolutionary mutation.Applied microbiology and biotechnology · 2025Article
- Engineering of L-threonine and L-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening.Bioresources and bioprocessing · 2025Article
- Construction and screening of L-valine high-yieldingFrontiers in microbiology · 2025Article
- Engineering of a Substrate Affinity Reduced S-Adenosyl-methionine Synthetase as a Novel Biosensor for Growth-Coupling Selection of L-Methionine Overproducers.Applied biochemistry and biotechnology · 2024Article
- Recent advances in screening amino acid overproducers.Engineering microbiology · 2023Review
- Minireview: Engineering evolution to reconfigure phenotypic traits in microbes for biotechnological applications.Computational and structural biotechnology journal · 2023Review
- Design and application of artificial rare L-lysine codons inFrontiers in bioengineering and biotechnology · 2023Article
- Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass inBiomolecules · 2022Review
- CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an L-proline exporter for L-proline hyperproduction.Nature communications · 2022Article
- Programming adaptive laboratory evolution of 4-hydroxyisoleucine production driven by a lysine biosensor in Corynebacterium glutamicum.AMB Express · 2021Article
- Tunable expression rate control of a growth-decoupled T7 expression system by L-arabinose only.Microbial cell factories · 2021Article
- Expression of transgenes enriched in rare codons is enhanced by the MAPK pathway.Scientific reports · 2020Article
- Significantly enhancing production ofScience advances · 2020Article
- Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli.Microbial cell factories · 2020Article
- EngineeredBiotechnology for biofuels · 2019Article
- Recent Advances of L-ornithine Biosynthesis in Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The translation of rare codons relies on their corresponding rare tRNAs, which could not be fully charged under amino acid starvation. Theoretically, disrupted or retarded translation caused by the lack of charged rare tRNAs can be partially restored by feeding or intracellular synthesis of the corresponding amino acids. Inspired by this assumption, we develop a screening or selection system for obtaining overproducers of a target amino acid by replacing its common codons with the corresponding synonymous rare alternative in the coding sequence of selected reporter proteins or antibiotic-resistant markers. Results show that integration of rare codons can inhibit gene translations in a frequency-dependent manner. As a proof-of-concept, Escherichia coli strains overproducing L-leucine, L-arginine or L-serine are successfully selected from random mutation libraries. The system is also applied to Corynebacterium glutamicum to screen out L-arginine overproducers. This strategy sheds new light on obtaining and understanding amino acid overproduction strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.