ArticleScientific reports2018
Imaging of Murine Whole Lung Fibrosis by Large Scale 3D Microscopy aided by Tissue Optical Clearing.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Multidimensional lung imaging: integrated preclinical platforms enabling the identification of translational biomarkers for pulmonary research.Respiratory research · 2026Review
- Machine learning for automated classification of lung collagen in a urethane-induced lung injury mouse model.Biomedical optics express · 2024Article
- 3DMOUSEneST: a volumetric label-free imaging method evaluating embryo-uterine interaction and decidualization efficacy.Development (Cambridge, England) · 2024Article
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- A roadmap for developing and engineeringBiophysics reviews · 2023Review
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- Uncovering the cytotoxic effects of air pollution with multi-modal imaging ofRoyal Society open science · 2023Review
- Optical clearing and testing of lung tissue using inhalation aerosols: prospects for monitoring the action of viral infections.Biophysical reviews · 2022Review
- Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies.Histochemistry and cell biology · 2022Review
- A simple optical tissue clearing pipeline for 3D vasculature imaging of the mediastinal organs in mice.International journal of experimental pathology · 2021Article
- Label-Free Multiphoton Microscopy: Much More Than Fancy Images.International journal of molecular sciences · 2021Review
- Tissue clearing technique: Recent progress and biomedical applications.Journal of anatomy · 2021Review
- New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution.Viruses · 2021Article
- Editorial: Pulmonary Hypertension in the Modern Era: Science and Clinical Practice.Frontiers in medicine · 2021Article
- An advanced optical clearing protocol allows label-free detection of tissue necrosisTheranostics · 2021Article
- Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In Vivo.Advances in experimental medicine and biology · 2021Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Self-organizing pattern of subpleural alveolar ducts.Scientific reports · 2020Article
- From 2D to 3D: Promising Advances in Imaging Lung Structure.Frontiers in medicine · 2020Article
- Targeting inducible epigenetic reprogramming pathways in chronic airway remodeling.Drugs in context · 2019Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Pulmonary fibrosis, characterized by excessive collagen deposition in the lungs, comprises a key and debilitating component of chronic lung diseases. Methods are lacking for the direct visualization of fibrillar collagen throughout the whole murine lung, a capability that would aid the understanding of lung fibrosis. We combined an optimized organ-level optical clearing (OC) approach with large-scale, label-free multiphoton microscopy (MPM) and second harmonic generation microscopy (SHGM) to reveal the complete network of fibrillar collagen in whole murine lungs. An innate inflammation-driven model based on repetitive poly(I:C) challenge was evaluated. Following OC, mosaic MPM/SHGM imaging with 3D reconstruction and whole organ quantitative analysis revealed significant differences in collagen deposition between PBS and poly(I:C) treated lungs. Airway specific analysis in whole lung acquisitions revealed significant sub-epithelial fibrosis evident throughout the proximal conductive and distal airways with higher collagen deposition in the poly(I:C) group vs PBS group. This study establishes a new, powerful approach based on OC and MPM/SHGM imaging for 3D analysis of lung fibrosis with macroscopic views of lung pathology based on microscopy and providing a new way to analyze the whole lung while avoiding regional sampling bias.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.