ArticleNature communications2018
Jungle Express is a versatile repressor system for tight transcriptional control.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- Identification of proteins influencing CRISPR-associated transposases for enhanced genome editing.Science advances · 2026Article
- Conserved Motifs in the Ligand-Binding Domain of TetR Family Regulators: Identification and Analysis.Computational and structural biotechnology journal · 2026Article
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- Genetically Encoded Control ofACS synthetic biology · 2025Article
- A rapid and efficient strategy for combinatorial repression of multiple genes in Escherichia coli.Microbial cell factories · 2025Article
- CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d.Nature microbiology · 2025Article
- Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida.Communications biology · 2025Article
- AND Logic Based on Suppressor tRNAs Enables Stringent Control of Sliding Base Editors inACS synthetic biology · 2024Article
- Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit in Pseudomonas aeruginosa.Nucleic acids research · 2024Article
- Tools for genetic engineering and gene expression control inApplied and environmental microbiology · 2024Article
- Controlling the expression of heterologous genes in Bdellovibrio bacteriovorus using synthetic biology strategies.Microbial biotechnology · 2024Article
- Snowprint: a predictive tool for genetic biosensor discovery.Communications biology · 2024Article
- Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit inbioRxiv : the preprint server for biology · 2024Article
- Review
- A New Mechanism of Carbon Metabolism and Acetic Acid Balance Regulated by CcpA.Microorganisms · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors.
Funding
Abstract
Tightly regulated promoters are essential for numerous biological applications, where strong inducibility, portability, and scalability are desirable. Current systems are often incompatible with large-scale fermentations due to high inducer costs and strict media requirements. Here, we describe the bottom-up engineering of 'Jungle Express', an expression system that enables efficient gene regulation in diverse proteobacteria. This system is guided by EilR, a multidrug-binding repressor with high affinity to its optimized operator and cationic dyes that act as powerful inducers at negligible costs. In E. coli, the engineered promoters exhibit minimal basal transcription and are inducible over four orders of magnitude by 1 µM crystal violet, reaching expression levels exceeding those of the strongest current bacterial systems. Further, we provide molecular insights into specific interactions of EilR with its operator and with two inducers. The versatility of Jungle Express opens the way for tightly controlled and efficient gene expression that is not restricted to host organism, substrate, or scale.
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