ArticleBMC plant biology2018
Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- The Evolution, Expression Patterns, and Domestication Selection Analysis of the Annexin Gene Family in the Barley Pan-Genome.International journal of molecular sciences · 2024Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- Comprehensive analysis of annexin gene family and its expression in response to branching architecture and salt stress in crape myrtle.BMC plant biology · 2024Article
- High temporal-resolution transcriptome landscapes of maize embryo sac and ovule during early seed development.Plant molecular biology · 2023Article
- ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution inPlants (Basel, Switzerland) · 2023Article
- Genome-Wide Characterization and Abiotic Stresses Expression Analysis of Annexin Family Genes in Poplar.International journal of molecular sciences · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- The durum wheat annexin, TdAnn6, improves salt and osmotic stress tolerance in Arabidopsis via modulation of antioxidant machinery.Protoplasma · 2021Article
- Characterization ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes inInternational journal of molecular sciences · 2021Article
- Overexpression of CassavaPlants (Basel, Switzerland) · 2021Article
- Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Comprehensive analyses of the annexin (ANN) gene family in Brassica rapa, Brassica oleracea and Brassica napus reveals their roles in stress response.Scientific reports · 2020Article
- Secretory Vesicles Targeted to Plasma Membrane During Pollen Germination and Tube Growth.Frontiers in cell and developmental biology · 2020Review
- Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundPollen development is a strictly controlled post-meiotic process during which microspores differentiate into microgametophytes and profound structural and functional changes occur in organelles. Annexin 5 is a calcium- and lipid-binding protein that is highly expressed in pollen grains and regulates pollen development and physiology. To gain further insights into the role of ANN5 in Arabidopsis development, we performed detailed phenotypic characterization of Arabidopsis plants with modified ANN5 levels. In addition, interaction partners and subcellular localization of ANN5 were analyzed to investigate potential functions of ANN5 at cellular level.
resultsHere, we report that RNAi-mediated suppression of ANN5 results in formation of smaller pollen grains, enhanced pollen lethality, and delayed pollen tube growth. ANN5 RNAi knockdown plants also displayed aberrant development during the transition from the vegetative to generative phase and during embryogenesis, reflected by delayed bolting time and reduced embryo size, respectively. At the subcellular level, ANN5 was delivered to the nucleus, nucleolus, and cytoplasm, and was frequently localized in plastid nucleoids, suggesting a likely role in interorganellar communication. Furthermore, ANN5-YFP co-immunoprecipitated with RABE1b, a putative GTPase, and interaction in planta was confirmed in plastidial nucleoids using FLIM-FRET analysis.
conclusionsOur findings let us to propose that ANN5 influences basal cell homeostasis via modulation of plastid activity during pollen maturation. We hypothesize that the role of ANN5 is to orchestrate the plastidial and nuclear genome activities via protein-protein interactions however not only in maturing pollen but also during the transition from the vegetative to the generative growth and seed development.
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