ArticleAngewandte Chemie (International ed. in English)2018
High-Density RNA Microarrays Synthesized In Situ by Photolithography.
Article in Angewandte Chemie (International ed. in English), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Scarless one-tube genome assemblyRSC chemical biology · 2026Article
- Synthesis of highly complex phosphorothioate-modified oligonucleotides on microarrays.Scientific reports · 2026Article
- Synthesis of long and functionally active RNAs facilitated by acetal levulinic ester chemistry.Nucleic acids research · 2026Article
- Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine OCommunications chemistry · 2025Article
- Accelerated, high-quality photolithographic synthesis of RNA microarrays in situ.Science advances · 2024Article
- Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates.Analytical chemistry · 2024Article
- Dipodal Silanes Greatly Stabilize Glass Surface Functionalization for DNA Microarray Synthesis and High-Throughput Biological Assays.Analytical chemistry · 2023Article
- Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays.Biomolecules · 2023Review
- Enzymatic Synthesis of High-Density RNA Microarrays.Current protocols · 2023Article
- Simple synthesis of massively parallel RNA microarrays via enzymatic conversion from DNA microarrays.Nature communications · 2022Article
- Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA.RSC advances · 2022Article
- Sequence Preference and Initiator Promiscuity forACS synthetic biology · 2021Article
- Chemical and photochemical error rates in light-directed synthesis of complex DNA libraries.Nucleic acids research · 2021Article
- Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors.ACS sensors · 2021Review
- l-DNA Duplex Formation as a Bioorthogonal Information Channel in Nucleic Acid-Based Surface Patterning.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Specificity and Efficiency of the Uracil DNA Glycosylase-Mediated Strand Cleavage Surveyed on Large Sequence Libraries.Scientific reports · 2019Article
- Large-Scale Photolithographic Synthesis of Chimeric DNA/RNA Hairpin Microarrays To Explore Sequence Specificity Landscapes of RNase HII Cleavage.Biochemistry · 2019Article
- Generation of small molecule-binding RNA arrays and their application to fluorogen-binding RNA aptamers.Methods (San Diego, Calif.) · 2019Article
- Multi-level patterning nucleic acid photolithography.Nature communications · 2019Article
- Spotting, Transcription andComputational and structural biotechnology journal · 2019Review
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4 authors.
Funding
Abstract
While high-density DNA microarrays have been available for over three decades, the synthesis of equivalent RNA microarrays has proven intractable until now. Herein we describe the first in situ synthesis of mixed-based, high-density RNA microarrays using photolithography and light-sensitive RNA phosphoramidites. With coupling efficiencies comparable to those of DNA monomers, RNA oligonucleotides at least 30 nucleotides long can now efficiently be prepared using modified phosphoramidite chemistry. A two-step deprotection route unmasks the phosphodiester, the exocyclic amines and the 2' hydroxyl. Hybridization and enzymatic assays validate the quality and the identity of the surface-bound RNA. We show that high-density is feasible by synthesizing a complex RNA permutation library with 262144 unique sequences. We also introduce DNA/RNA chimeric microarrays and explore their applications by mapping the sequence specificity of RNase HII.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.