Evidence map›Paper›PMID 30187339›Full record

ArticleInflammation2019

CDK5 Regulates PD-L1 Expression and Cell Maturation in Dendritic Cells of CRSwNP.

C C Liu, H L Zhang, L L Zhi, P Jin, L Zhao, T Li, X M Zhou, D S Sun, G H Cheng, Q Xin and 2 more

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Inflammation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 8 citations in OpenAlex.

  1. Pooled it
  2. Immunological Aspects of Chronic Rhinosinusitis.Diagnostics (Basel, Switzerland) · 2022
    Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

C C LiuDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China.
H L ZhangDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China.
L L ZhiDepartment of Otolaryngology, The Central Hospital of Zibo, No.54, Gongqingtuan West Road, Zhangdian District, Zibo, Shandong Province, China.
P JinDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China.
L ZhaoDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China.
T LiDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China.
X M ZhouDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China.
D S SunCentral Laboratory, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, Shandong Province, China.
G H ChengDepartment of Cancer Center, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, Shandong Province, China.
Q XinDepartment of Cancer Center, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, Shandong Province, China.
L ShiDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China. shili126@sina.com.
M XiaDepartment of Otolaryngology, The Second Hospital of Shandong University, Shandong University, No.274 Beiyuan Road, Jinan, 250033, Shandong Province, China. xiamingsdu@sohu.com.
Second Hospital of Shandong University · CNCentral Hospital of Zibo · CN

Funding

Clinical medical science and technology innovation plan of Jinan City 201506005The key research and development plan project of Shandong 2016GSF201077The key research and development plan project of Shandong 2016GSF201084The key research and development plan project of Shandong 2016GSF201140Youth Talent Fund of Second Hospital of Shandong university 2018YT12
6 · The paper itself

Abstract

The maturation of dendritic cells is critical for chronic rhinosinusitis with nasal polyps (CRSwNPs), especially eosinophilic chronic rhinosinusitis with nasal polyps (EosCRSwNPs), but the regulation mechanism of dendritic cells (DCs) maturation is still unclear. We identified nasal mucosa of 20 patients with EosCRSwNP, 16 non-EosCRSwNP patients, and inferior turbinate of 14 patients with nasal septum deviation after surgery. The expression of cyclin-dependent kinase 5 (CDK5) and programmed cell death 1 ligand 1 (PD-L1) were detected by immunofluorescent, real-time quantitative PCR, and Western blot in EosCRSwNP. The level of dendritic cell maturation was detected by flow cytometry and immunofluorescence staining after CDK5 expression interference with small interfering RNA (siRNA). The expression of CDK5 and PD-L1 in EosCRSwNP nasal mucosal tissue was significantly higher than that of non-EosCRSwNP and inferior turbinate nasal mucosa tissue, and there was a positive correlation between them. Immunofluorescence staining showed that CDK5 and PD-L1 were co-localized in dendritic cells. Synergistic stimulation of dendritic cells with LPS and TNF-α promotes the maturation of dendritic cells and increases the expression of CDK5 and PD-L1. However, blocking the expression of CDK5 in dendritic cells with siRNAs leads to a blockage of cell maturation. CDK5 can regulate the expression of PD-L1, and its presence is critical for the maturation of dendritic cells. CDK5 may play an important role in the pathogenesis of CRSwNP disease.

Indexed as

B7-H1 AntigenCell DifferentiationChronic DiseaseCyclin-Dependent Kinase 5Dendritic CellsHumansLipopolysaccharidesNasal MucosaNasal PolypsRhinitisSinusitisTumor Necrosis Factor-alphaB7-H1 AntigenCD274 protein, humanCDK5 protein, humanCyclin-Dependent Kinase 5LipopolysaccharidesTumor Necrosis Factor-alphaCDK5CRSwNPdendritic cellsinflammatorymaturationPD-L1

Identifiers

PMID30187339
OpenAlexW2890181233

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.