ArticleInfection2019
Clinical benefits of introducing real-time multiplex PCR for cerebrospinal fluid as routine diagnostic at a tertiary care pediatric center.
Article in Infection, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- Review
- Clinical utility of a cerebrospinal fluid multiplex PCR meningitis/encephalitis panel in children: a retrospective review of positive results.BMC infectious diseases · 2026Article
- The Use of CSF Multiplex PCR Panel in Patients with Viral Uveitis.Diagnostics (Basel, Switzerland) · 2026Article
- Does cerebrospinal fluid multiplex polymerase chain reaction meningitis panel help manage children with meningitis?Research square · 2025Article
- Clinical utility of the FilmArray® meningitis/encephalitis panel in children with suspected central nervous system infection in a low-resource setting - a prospective study in Southwestern Uganda.BMC infectious diseases · 2025Observational
- The role of rapid multiplex molecular syndromic panels in the clinical management of infections in critically ill patients: an experts-opinion document.Critical care (London, England) · 2024Review
- Performance Evaluation of NovaplexMicroorganisms · 2024Article
- Clinical course and peculiarities of Parechovirus and Enterovirus central nervous system infections in newborns: a single-center experience.European journal of pediatrics · 2024Article
- Article
- Human parechovirus meningitis in children: state of the art.Italian journal of pediatrics · 2023Review
- Detection of enterovirus in cerebrospinal fluids without pleocytosis in febrile infants under 3 months old reduces antibiotherapy duration.Frontiers in pediatrics · 2023Article
- Current state and practice variation in the use of Meningitis/Encephalitis (ME) FilmArray panel in children.BMC infectious diseases · 2022Article
- Biofire FilmArray Meningitis/Encephalitis panel for the aetiological diagnosis of central nervous system infections: A systematic review and diagnostic test accuracy meta-analysis.EClinicalMedicine · 2022Article
- A discussion of syndromic molecular testing for clinical care.The Journal of antimicrobial chemotherapy · 2021Article
- Small-volume detection: platform developments for clinically-relevant applications.Journal of nanobiotechnology · 2021Review
- Prospective case-control study of enterovirus detection differences in children's cerebrospinal fluid between multiplex PCR and real-time RT-PCR assay.Journal of clinical laboratory analysis · 2021Article
- Impact of cerebrospinal fluid syndromic testing in the management of children with suspected central nervous system infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Varicella Vaccine Meningitis as a Complication of Herpes Zoster in Twice-Immunized Immunocompetent Adolescents.Journal of child neurology · 2020Article
- Rapid Diagnostic Tests for Meningitis and Encephalitis-BioFire.Pediatric emergency care · 2020Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSepsis-like illness with suspected meningitis or encephalitis is a common reason for using empiric antimicrobial therapy in infants and children. However, in cases of viral meningitis not covered by these antimicrobials, this management is ineffective and due to side effects potentially harmful.
methodsA retrospective analysis of cerebrospinal fluid (CSF) multiplex PCRs (Biofire FilmArray
resultsDuring the study period, the multiplex PCR panel was performed on 187 individual CSF samples that met the inclusion criteria. About half of the patients (92/187) were less than 1 year of age. In 27 cases (14.4%), the PCR yielded a positive result with the majority (12/27) being indicative of an enteroviral infection. In the age group of 8-84 days of life, 36.4% of the patients had a positive result. When the patients with a PCR positive for a viral agent were compared to an age-matched group of patients, no differences were observed regarding symptoms and laboratory parameters. However, the duration of antimicrobial therapy could be significantly reduced through the use of multiplex PCR.
conclusionThe use of on-site diagnostic multiplex PCR was able to reduce the use of antimicrobials in selected cases. This test can guide clinical decisions earlier during the course of medical care compared to standard diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.