Evidence map›Paper›PMID 30186835›Full record

ArticleFrontiers in cell and developmental biology2018

Multiplexed CRISPR/Cas9 Targeting of Genes Implicated in Retinal Regeneration and Degeneration.

Arife Unal Eroglu, Timothy S Mulligan, Liyun Zhang, David T White, Sumitra Sengupta, Cathy Nie, Noela Y Lu, Jiang Qian, Lisha Xu, Wuhong Pei and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cell and developmental biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Review
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  4. CRISPR-based functional genomics tools in vertebrate models.Experimental & molecular medicine · 2025
    Review
  5. Article
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  8. Article
  9. Article
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  11. Article
  12. Article
  13. The application of genome editing technology in fish.Marine life science & technology · 2021
    Review
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  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Arife Unal ErogluDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Timothy S MulliganDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Liyun ZhangDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
David T WhiteDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Sumitra SenguptaDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Cathy NieDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Noela Y LuDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Jiang QianDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Lisha XuTranslational and Functional Genomics Branch, National Human Genome Research Institute, Bethesda, MD, United States.
Wuhong PeiTranslational and Functional Genomics Branch, National Human Genome Research Institute, Bethesda, MD, United States.
Shawn M BurgessTranslational and Functional Genomics Branch, National Human Genome Research Institute, Bethesda, MD, United States.
Meera T SaxenaDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Jeff S MummDepartment of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Johns Hopkins University · USJohns Hopkins Medicine · USNational Human Genome Research Institute · US

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Ji Yi · 1985 to 2026
$22.1M
Hearing regeneration in zebrafishZIAHG200386 · NHGRI · NATIONAL HUMAN GENOME RESEARCH INSTITUTE · PI BURGESS, SHAWN M · 2012 to 2025
$12.5M
Visual Science Training ProgramT32EY007143 · NEI · JOHNS HOPKINS UNIVERSITY · PI Laura Ensign, JEREMY NATHANS · 1995 to 2026
$6.2M
Genetic and Chemical Screens for Factors Regulating Retinal RegenerationR01EY022810 · NEI · JOHNS HOPKINS UNIVERSITY · PI MUMM, JEFFREY · 2013 to 2017
$2.1M
Robotic whole organism HTS platform for drug discovery and developmentR41TR000945 · NCATS · LUMINOMICS, INC. · PI MUMM, JEFFREY · 2014 to 2014
$225k
Intramural NIH HHS ZIA HG200386NCATS NIH HHS R41 TR000945NEI NIH HHS P30 EY001765NEI NIH HHS R01 EY022810NEI NIH HHS T32 EY007143
6 · The paper itself

Abstract

Thousands of genes have been implicated in retinal regeneration, but only a few have been shown to impact the regenerative capacity of Müller glia-an adult retinal stem cell with untapped therapeutic potential. Similarly, among nearly 300 genetic loci associated with human retinal disease, the majority remain untested in animal models. To address the large-scale nature of these problems, we are applying CRISPR/Cas9-based genome modification strategies in zebrafish to target over 300 genes implicated in retinal regeneration or degeneration. Our intent is to enable large-scale reverse genetic screens by applying a multiplexed gene disruption strategy that markedly increases the efficiency of the screening process. To facilitate large-scale phenotyping, we incorporate an automated reporter quantification-based assay to identify cellular degeneration and regeneration-deficient phenotypes in transgenic fish. Multiplexed gene targeting strategies can address mismatches in scale between "big data" bioinformatics and wet lab experimental capacities, a critical shortfall limiting comprehensive functional analyses of factors implicated in ever-expanding multiomics datasets. This report details the progress we have made to date with a multiplexed CRISPR/Cas9-based gene targeting strategy and discusses how the methodologies applied can further our understanding of the genes that predispose to retinal degenerative disease and which control the regenerative capacity of retinal Müller glia cells.

Indexed as

CRISPR/Cas9large scalemultiplexregenerationretinaretinal degenerationrhodopsinzebrafish

Identifiers

PMID30186835
PMCPMC6111214
OpenAlexW2888135435

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.