ArticleFrontiers in chemistry2018
UPLC-MS/MS Based Identification of Dietary Steryl Glucosides by Investigation of Corresponding Free Sterols.
Article in Frontiers in chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Free Sterol Content ofMolecules (Basel, Switzerland) · 2026Article
- Microbial biopriming and germination cooperatively remodel brown lentil seeds (Lens culinaris L.) metabolome and antidiabetic functionality.NPJ science of food · 2026Article
- Molecular Insights of Neuroprotective Effect ofInternational journal of molecular sciences · 2026Article
- Unveiling crucifer metabolomes via UPLC-HRMS/MS and chemometric analysis of edible and non-edible varieties.Scientific reports · 2025Article
- Diagnostic Fragmentations of Animal and Fungal Sterols/Stanols Obtained by APCI-Tandem Mass Spectrometry: A Route Towards Unknown Free Sterol Identification.Metabolites · 2025Article
- APCI-Multistage Mass Spectrometry Following Liquid Chromatography for Selected 4-Desmethyl-Sterols and Their Deuterium-Labelled Analogues Unveils Characteristic Fragmentation Routes for Cholesterol and Phytosterols Identification.Rapid communications in mass spectrometry : RCM · 2025Article
- Vacuolar sterol β-glucosidase EGCrP2/Sgl1 deficiency in Cryptococcus neoformans: Dysfunctional autophagy and Mincle-dependent immune activation as targets of novel antifungal strategies.PLoS pathogens · 2025Article
- Virgin Olive oil Authenticity Assays in a Single Run Using Two-Dimensional Liquid Chromatography-High Resolution Mass Spectrometry.Analytical chemistry · 2024Article
- Chemical and Biological Characterization of the Ethyl Acetate Fraction from the Red Sea Marine SpongePharmaceuticals (Basel, Switzerland) · 2024Article
- Metabolomic Profiling and In Vivo Antiepileptic Effect ofMetabolites · 2024Article
- A lipidome landscape of aging in mice.Nature aging · 2024Article
- Chemical and Biochemical Features of Spinasterol and Schottenol.Advances in experimental medicine and biology · 2024Article
- Characterization of thraustochytrid-specific sterolApplied and environmental microbiology · 2023Article
- Advances and Challenges in Plant Sterol Research: Fundamentals, Analysis, Applications and Production.Molecules (Basel, Switzerland) · 2023Review
- Characterization of the Sterol 24-C-Methyltransferase Genes Reveals a Network of Alternative Sterol Biosynthetic Pathways inMicrobiology spectrum · 2023Article
- Optimization of Magnetic and Paper-Based Molecularly Imprinted Polymers for Selective Extraction of Charantin inInternational journal of molecular sciences · 2023Article
- Isolation, Physicochemical Characterization, and Biological Properties of Inotodiol, the Potent Pharmaceutical Oxysterol from Chaga Mushroom.Antioxidants (Basel, Switzerland) · 2023Article
- New Structures, Spectrometric Quantification, and Inhibitory Properties of Cardenolides fromMolecules (Basel, Switzerland) · 2022Article
- Comparative Assessment of the Antioxidant and Anticancer Activities ofAntioxidants (Basel, Switzerland) · 2022Article
- Identification and Distribution of Sterols, Bile Acids, and Acylcarnitines by LC-MS/MS in Humans, Mice, and Pigs-A Qualitative Analysis.Metabolites · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dietary plant foods are characterized by a vast molecular diversity of glycosylated sterols (SG) that differ in the structure of the steryl backbone. The identification of these polar steryl conjugates represents a major challenge as they are structurally highly similar, and commercial standards are limited to a few naturally abundant species. Spectral databases do not yet contain MS/MS spectra of these sterol conjugates obtained by electrospray ionization (ESI), which would facilitate their reliable identification. Thus, this study aimed at providing novel information on ESI-MS/MS spectra of both abundant and minor SG found in foods. As a first step, however, free sterols (FS) were investigated for their fragmentation behavior as they share the same intermediate ion as SG. Pure SG were obtained from commercially available standard mixtures and minor SG were extracted from different food sources (oat bran, wheat bran, pumpkin seeds, melon, rapeseeds, and potato peel). ESI-MS/MS spectra of 15 FS were assessed and fragment ions reflective of structural features were identified and rationalized. Subsequently, 14 SG were identified at four different levels, while relative retention times from chromatographic separation and spectral features of FS served to identify five SG. Spectral data from FS were directly transferable to SG when analyzed as aglycone ions as shown by similarity scores while SG were characterized by shorter retention times in reverse phase chromatography and the additional analysis as sodiated adduct confirmed their glycosidic nature. Moreover, we report for the first time the occurrence of 24-methylenecholesterol and a 4-monomethyl sterol as glycosidic conjugates in higher plants. The presented data will serve as a valuable tool for SG profiling of foods by facilitating their identification.
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